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Obsolete

Part Number:
600151
RUO

Obsolete. No replacement recommendation. SP6 RNA Polymerase. Contains: Reaction and dilution buffers, Concentration is 50 units/µL

For Research Use Only. Not for use in diagnostic procedures.
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Specifications
Concentration
  • 50 U/µL
Incubation Time
  • Incubate 15 minutes at 37 °C
Purity
  • 0.99 %
Reaction Conditions
  • 40mM Tris-HCl (pH 8.0), 8 mM MgCl2, 50 mM NaCl, 2 mM spermidine, 1 mM DTT, .4mM (ATP,GTP, CTP, UTP), 1microCurie alpha-32P UTP, 2ug linearized SP6 vector and 10 units enzyme in a 100 µL volume
Reactions per Kit
  • 3000 units
Storage Buffer
  • 40 mM Tris-HCl (pH 8.0), 8 mM MgCl2, 50 mM NaCl, 2 mM spermidine, 30 mM DTT, 400 µM of each rNTP, 1 µg DNA template and 20 units enzyme in a 25 µl volume. Incubate 60 minutes at 37°C.
Unit Definition
  • One unit will catalyze the incorporation of 1 nmole of nucleoside triphosphate into an acid insoluble form in 60 minutes at 37°C.