GKX-5003
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Exoglycosidases

Part Number:
GKX-5003

β-N-Acetylhexosaminidase (jack bean). Enzyme releases nonreducing terminal β(1-2,3,4,6)-linked N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc) residues. Includes 5x Incubation Buffer [500 mM sodium citrate phosphate (pH 5.0)] 

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List Price:
$274.00

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Specifications
Concentration
  • 50 U/mL
Enzyme Applications
  • Useful for the structural analysis of isolated glycans and glycoconjugates. pH optimum/range: For glucosaminide 5.0 - 6.0, For galactosaminide 3.5 - 4.0
Enzyme Formulation
  • 20mM sodium citrate phosphate (pH 6.0)
Enzyme Source
  • Jack bean.
Enzyme Specificity
  • The enzyme exhibits a broad specificity, cleaving nonreducing terminal ß(1-2,3,4,6)-linked N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc) residues. This enzyme is useful in the study of isolated glycans, glycolipids and glycoproteins, especially in combination with ß N-acetylhexosaminidase from S. pneumoniae (GK80050). We also offer this enzyme at a higher concentration - see GKX-5023. Higher enzyme concentrations may be necessary to remove bisecting ß(1-4)-linked GlcNAc residues in complex N-glycans.
Enzyme Unit Definition
  • One unit is defined as the amount of enzyme required to hydrolyze 1 µmole of pNP N-acetyl-ß-D-glucosaminide per minute at pH 5.0 and 37°C.
Molecular Weight
  • 100 - 110 kDa
Unit
  • 5 U
Volume
  • 100 µL
Support
Safety Data Sheets

β-N-Acetylhexosaminidase (jack bean)

β-N-Acetylhexosaminidase (jack bean), 100 μl, 5x Reaction Buffer, 1 ml

  • Safety Data Sheets
  • English
  • 15-Jul-2019
  • 450.83 Kb
  • PDF

β-N-Acetylhexosaminidase (jack bean)

β-N-Acetylhexosaminidase (jack bean), 100 μl, 5x Reaction Buffer, 1 ml

  • Safety Data Sheets
  • English
  • 15-Jul-2019
  • 441.36 Kb
  • PDF

β-N-Acetylhexosaminidase (jack bean)

β-N-Acetylhexosaminidase (jack bean), 100 μl, 5x Reaction Buffer, 1 ml

  • Safety Data Sheets
  • English
  • 15-Jul-2019
  • 497.37 Kb
  • PDF

β-N-Acetylhexosaminidase (jack bean)

β-N-Acetylhexosaminidase (jack bean), 100 μl, 5x Reaction Buffer, 1 ml

  • Safety Data Sheets
  • English
  • 15-Jul-2019
  • 438.64 Kb
  • PDF

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