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Endoglycosidases

Part Number:
GKE-5020B-5

AdvanceBio N-Glycanase-ultra (PNGase F, EDTA free), ≥10 U/mL. A recombinant form of PNGase F, releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. The same formulation provided in GlykoPrep, ≥ 10 U/mL and EDTA-free. Supplied as 5 x 400 mU vials of GKE-5020B. Includes 5x N-glycanase reaction buffer, denaturation solution, detergent solution.

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Specifications
Concentration
  • 10 U/mL
Enzyme Applications
  • To obtain efficient deglycosylation of glycoprotein substrates under non-denaturing conditions, it is necessary to use a higher starting concentration of enzyme. N-Glycanase PLUS and ULTRA (EDTA-Free) are supplied at = 10 U/ml, and are recommended for all applications requiring deglycosylation of glycoproteins in the absence of denaturants. The high activity also allows smaller reaction volumes and shorter reaction times to be explored.
Enzyme Formulation
  • 20 mM Tris-HCl, 50 mM NaCl (pH 7.5, EDTA-free formulation).
Enzyme Source
  • Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as Endoglycosidase F2, endo-beta-N-acetylglucosaminidase. Enzyme also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase.
Enzyme Specific Activity
  • ≥10 units/mg
Enzyme Specificity
  • Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless a(1-3) core fucosylated, as in plant glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate and sialic, acid groups attached to the oligosaccharide do not affect cleavage. Endo F free. Highly concentrated, particularly useful for deglycosylation under native conditions.
Enzyme Unit Definition
  • One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 µmole of denatured ribonuclease B per minute at 37°C, pH 7.5.
Molecular Weight
  • ~35,000 Daltons
Unit
  • 2000 mU
Volume
  • 400 µL
pH Optimum
  • 8.6
pH Range
  • 7.5-9.5
Literature
Data Sheets

Agilent AdvanceBio N-Glycanase-ULTRA

Agilent AdvanceBio N-Glycanase ULTRA for the removal of N-glycans from glycoproteins and glycopeptides.

  • Data Sheets
  • English
  • 30-Jul-2019
  • 571.71 Kb
  • PDF
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Safety Data Sheets

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

  • Safety Data Sheets
  • English
  • 12-May-2019
  • 69.05 Kb
  • PDF

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

  • Safety Data Sheets
  • English
  • 12-May-2019
  • 170.32 Kb
  • PDF

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

  • Safety Data Sheets
  • English
  • 12-May-2019
  • 166.62 Kb
  • PDF

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

  • Safety Data Sheets
  • English
  • 12-May-2019
  • 198.08 Kb
  • PDF

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