GKE-5016A
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Endoglycosidases

Part Number:
GKE-5016A

AdvanceBio N-Glycanase (PNGase F, EDTA free), ≥2.5 U/mL (formerly ProZyme). A recombinant form of PNGase F (100 mU, EDTA free), releases intact N-glycans by cleaving between the innermost GlcNAc and Asn.  Includes 5x N-glycanase reaction buffer, denaturation solution, detergent solution, 5x N-glycanase Tris reaction buffer (MS applications).

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List Price:
$276.00

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Specifications
Concentration
  • 2.5 U/mL
Enzyme Formulation
  • 20 mM Tris-HCl, 50 mM NaCl, (pH 7.5)
Enzyme Source
  • Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as Endoglycosidase F2, endo-beta-N-acetylglucosaminidase. Enzyme also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase.
Enzyme Specific Activity
  • ≥10 units/mg
Enzyme Specificity
  • Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless a(1-3) core fucosylated, as in plant glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate and sialic acid groups attached to the oligosaccharide do not affect cleavage. Endo F free. Highly concentrated, particularly useful for deglycosylation under native conditions.
Enzyme Unit Definition
  • One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 µmole of denatured ribonuclease B per minute at 37°C, pH 7.5.
Molecular Weight
  • ~35,000 Daltons
Unit
  • 100 mU
Volume
  • 40 µL
pH Optimum
  • 8.6
pH Range
  • 7.5-9.5
Literature
Data Sheets

Agilent AdvanceBio N-Glycanase

Agilent AdvanceBio N-Glycanase for the release of intact N-linked glycans from glycopeptides and glycoproteins and structure-function studies of N-glycosylated glycoproteins.

Support
Safety Data Sheets

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml