GKE-5002
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Endoglycosidases

Part Number:
GKE-5002

AdvanceBio Endoglycosidase H (formerly ProZyme). Enzyme cleaves within the N,N'-diacetylchitobiose (GlcNAc-GlcNAc) core of high mannose and some hybrid type N-glycans. Includes  5x reaction buffer for GKE-5002 (500 mM Sodium Citrate/Phosphate, pH 5.5, denaturation solution (2% SDS/1M βME, detergent solution (15% detergent solution).

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List Price:
$320.00

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Specifications
Application
  • Analysis of biosynthesis of glycoproteins
Concentration
  • ≥0.15 U/mL
Enzyme Applications
  • Selective release of high mannose N-linked oligosaccharides from glycopeptides/ glycoproteins. The enzyme has found extensive use in the characterization of glycoproteins, dolichol-linked glycans and the biosynthetic pathway for N-glycosylation.
Enzyme Formulation
  • 50 mM sodium phosphate pH 7.5, 25 mM EDTA, and 0.05% sodium azide
Enzyme Source
  • Recombinant gene from Streptomyces plicatus, expressed in E. coli. Enzyme is also known as Endoglycosidase H, mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase.
Enzyme Unit Definition
  • One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 60 µmoles of denatured ribonuclease B in 1 hour at 37°C, pH 5.5.
Unit
  • 30 mU
Volume
  • 200 µL
pH Optimum
  • 5.5
Support
Safety Data Sheets

AdvanceBio Endoglycosidase H

AdvanceBio Endoglycosidase H, 30 mU, Denaturation Solution, 500 μl, Detergent Solution, 200 μl, 5x Reaction Buffer, 1 ml

AdvanceBio Endoglycosidase H

AdvanceBio Endoglycosidase H, 30 mU, Denaturation Solution, 500 μl, Detergent Solution, 200 μl, 5x Reaction Buffer, 1 ml

AdvanceBio Endoglycosidase H

AdvanceBio Endoglycosidase H, 30 mU, Denaturation Solution, 500 μl, Detergent Solution, 200 μl, 5x Reaction Buffer, 1 ml

Certificate of Analysis

GKE-5002, GLYKO ENDOGLYCOSIDASE H (recombinant from Streptomycesplicatus, expressed in E. coli), 159 008a-1, Certificate of Analysis

GKE-5002, GLYKO ENDOGLYCOSIDASE H (recombinant from Streptomycesplicatus, expressed in E. coli), 159 008a-1, Certificate of Analysis