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Exoglycosidases

Part Number:
GK80046

AdvanceBio Sialidase A, 66 kDa. A lower molecular weight version of GK80040 sialidase A for challenging digestions. Enzyme releases α(2-3)-, α(2-6)-, α(2-8), and α(2-9)-linked N-acetylneuraminic acid from oligosaccharides and glycoproteins. Also capable of releasing N-glycolylneuraminic acid. Lower mol wt sialidases may help desialylate glycans if steric hindrance is an issue. If you think a lower mol wt sialidase will help desialylate your glycans, you can also try our 88, 66 and 51 kDa Sialidase A sampler kit (GK80043). 

Sialidases are also known as neuraminidases.

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List Price:
$343.00

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Specifications
Concentration
  • ≥5 U/mL
Enzyme Source
  • Recombinant gene from Arthrobacter ureafaciens, expressed in E. coli. Sialidases are also known as neuraminidases.
Enzyme Specificity
  • The enzyme releases a(2,3)-, a(2,6)-, a(2,8)-, and a(2,9)-linked N-acetylneuraminic acid from complex carbohydrates. The initial rate of hydrolysis of a(2,6) linkages is reported to be approximately twice that of a(2,3)-linked sialic acid however, in practice, this kinetic selectivity is of little consequence during extended incubations. Effective digestion of glycolipid substrates is facilitated by addition of a detergent, such as sodium taurodeoxycholate to the incubation. Sialidase A is capable of releasing N-glycolylneuraminic acid (Neu5Gc, NGNA) in addition to N-acetylneuraminic acid (Neu5Ac, NANA) [1], although similarly to other sialidases [2] the activity is lower toward Neu5Gc than Neu5Ac. The enzyme can also be used to remove sialic acid from gangliosides [3], glycosphingolipids (ceramide and oligosaccharide) with sialic acid. May be used for exoglycosidase sequencing with Sialidase S (GK80021), which is specific for a(2,3)-linked sialic acids. Sialidases are also known as neuraminidase.
Enzyme Unit Definition
  • One unit is defined as the amount of enzyme required to catalyze the release of 1 µmole of p-nitrophenol from p-nitrophenyl-α-D-N-acetylneuraminic acid per minute at 37° C, pH 5.5
Molecular Weight
  • 66 kDa
Unit
  • 1 U
Volume
  • 200 µL
pH Optimum
  • 6
Support
Safety Data Sheets

AdvanceBio Sialidase A 66, 5x Reaction Buffer B

AdvanceBio Sialidase A 66, 1 Unit (200 μl), 5x Reaction Buffer B, 1 ml

  • Safety Data Sheets
  • English
  • 15-Jul-2019
  • 335.18 Kb
  • PDF

AdvanceBio Sialidase A 66, 5x Reaction Buffer B

AdvanceBio Sialidase A 66, 1 Unit (200 μl), 5x Reaction Buffer B, 1 ml

  • Safety Data Sheets
  • English
  • 15-Jul-2019
  • 325.17 Kb
  • PDF

AdvanceBio Sialidase A 66, 5x Reaction Buffer B

AdvanceBio Sialidase A 66, 1 Unit (200 μl), 5x Reaction Buffer B, 1 ml

  • Safety Data Sheets
  • English
  • 15-Jul-2019
  • 322.99 Kb
  • PDF

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