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Pfu DNA Ligase

Part Number:
600191
RUO

Pfu DNA Ligase. Kit contains: 400 U Pfu DNA Ligase 10X reaction buffer

For Research Use Only. Not for use in diagnostic procedures.
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List Price:
$502.00 /10

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Specifications
Concentration
  • 4 U/µL
Inactivation
  • Must be removed by protein extraction methods
Ligase Type
  • Pfu DNA ligase
Quantity per Kit
  • 400 U
Reaction Conditions
  • 4 U of Pfu DNA ligase enzyme, in 20 mM Tris-HCl (pH 7.5), 20 mM KCl, 10 mM MgCl2, 0.1 % Igepal, 0.01 mM rATP, 1 mM DTT
Reactions per Kit
  • 400 units
Storage Buffer
  • 50 mM Tris-HCl (pH 7.5), 50 % glycerol, 1 mM EDTA, 10 mM DTT, 0.1 % Tween 20, 0.1 % Igepal
Unit Definition
  • One unit will ligate to completion 0.5 µg of nicked pBluescript DNA in 15 minutes at 55°C.