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Safety Data Sheets
Seahorse XF Cell Mito Stress Test Kit (PDF)
  • Released: 22 May 2025
  • 196 KB
  • PDF
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User Manuals
Agilent Seahorse XF 3D Mito Stress Test Assay User Guide (PDF)

User manual for the Seahorse XF 3D Mito Stress Test Assay.

  • Publication Part Number: 5994-8177EN
  • Created: 22 May 2025
  • 579 KB
  • PDF
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Applications
Measure Mitochondrial Respiration in Skeletal Muscle Fibers with a Seahorse Analyzer (PDF)

Measuring Mitochondrial Respiration in Intact Skeletal Muscle Fibers with an Agilent Seahorse XF24/XFe24 Analyzer

  • Publication Part Number: 5991-7148EN
  • Created: 22 Dec 2016
  • 1 MB
  • PDF
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Safety Data Sheets
Seahorse XF 3D Mito Stress Test Kit (PDF)
  • Released: 20 Feb 2025
  • 198 KB
  • PDF
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Safety Data Sheets
Seahorse XFp Cell Mito Stress Test Kit, Part Number 103010-100 (PDF)
  • Released: 05 Dec 2023
  • 152 KB
  • PDF
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Applications
Assessing therapies for traumatic brain or spinal cord injuries (PDF)

XF technology provides insight into treatment interventions for mitochondrial dysfunction

  • Publication Part Number: 5991-7126EN
  • Created: 22 Dec 2016
  • 680 KB
  • PDF
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Applications
Assessing the impact of autophagy on cellular metabolism (PDF)

Real-time, functional metabolic assays connect energy pathways and autophagy

  • Publication Part Number: 5991-7125EN
  • Created: 22 Dec 2016
  • 1 MB
  • PDF
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User Manuals
FCCP optimization using the Agilent Seahorse XF Cell Mito Stress Test with XF Pro Analyzer (PDF)

This document provides instruction on how to perform FCCP optimization using the Agilent Seahorse XF Cell Mito Stress Test with the Agilent Seahorse XF Pro Analyzer

  • Publication Part Number: 5994-4807EN
  • Created: 29 Apr 2022
  • 367 KB
  • PDF
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Technical Overviews
3T3-L1 Pre-Adipocyte Differentiation (PDF)

Characterize metabolic functionality of adipocytes differentiated from the pre-adipocyte 3Y3-L1 cell culture line using the Agilent Seahorse XF/XFe Analyzer.

  • Publication Part Number: 5991-7152EN
  • Created: 01 Oct 2020
  • 639 KB
  • PDF
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Applications
Identifying Metabolic Switches Using an Agilent Seahorse XFe Analyzer in Hypoxia (PDF)

Identifying Metabolic Phenotype Switches in Cancer Cells Using the Agilent Seahorse XF/XFe Analyzer in an Hypoxic Environment

  • Publication Part Number: 5991-7146EN
  • Created: 03 Jan 2017
  • 744 KB
  • PDF
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Publication
Hypoxia Prevents Mitochondrial Dysfunction and Senescence in Human c-Kit(+) Cardiac Progenitor Cells
  • Journal: Stem Cells
  • Publication Date: 10 Jan 2019
  • Author: Korski KI , et al.
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Authors K I Korski, D A Kubli, B J Wang, F G Khalafalla, M M Monsanto, F Firouzi, O H Echeagaray, T Kim, R M Adamson, W P Dembitsky, A B Gustafsson, and M A Sussman
Journal Stem Cells
Publication Date 10 Jan 2019
Research Area(s) include Assays Under Hypoxia, Cardiovascular Research, Stem Cell Biology
Technique Seahorse XF Technology
Analyzer(s) include Seahorse XFp
Mode N/A
Cell Line(s) include Cardiac stem cells
Cell Type(s) include Stem cells
Species Human
Assay / Method Cell Mitochondrial Stress Test
Cell Seeding Density 1.5x10^3 cells/well
Plate Coating Not Specified
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Applications
直接测量细胞代谢以识别线粒体药物靶点 (PDF)

概述安捷伦 Seahorse XF 细胞线粒体压力测试和XF PMP工作流程如何应用于线粒体和代谢靶点的识别研究。

  • Publication Part Number: 5994-0454ZHCN
  • Created: 30 Jan 2019
  • 516 KB
  • PDF
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Flyer
Seahorse XF Cell Mito Stress Test Kit (PDF)

A complete profile of mitochondrial function with the Seahorse XF Cell Mito Stress Test Kit

  • Publication Part Number: 5991-7118EN
  • Created: 01 Oct 2020
  • 2 MB
  • PDF
Supporting Documents
Chinese (Simplified) (China) Complete (PDF)
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Flyer
Agilent Seahorse XF 3D Mito Stress Test Kit flyer (PDF)

2025 flyer for the Agilent Seahorse XF 3D Mito Stress Test Kit for a comprehensive profile of mitochondrial function in 3D models.

  • Publication Part Number: 5994-8251EN
  • Created: 15 Apr 2025
  • 235 KB
  • PDF
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Posters
Multimodal Connected Workflow for Cardiotoxicity Assessment Using Human iPSC-Derived Cardiomyocytes (PDF)

The poster introduces a multiparametric workflow that allows for evaluating contractility, viability, and metabolic function using validated human iPSC-derived cardiomyocytes to better understand the mechanisms associated with drug-induced...

  • Publication Part Number: SOT # 4373
  • Created: 24 Mar 2025
  • 504 KB
  • PDF
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Posters
Metabolic Discovery Platform: Seahorse XF Analyzer with Bravo Liquid Handler (PDF)

This Agilent-CELLOMET collaboration demonstrates the quality and efficiency of coupling the Agilent Seahorse XFe96 Analyzer with the Agilent Bravo liquid handler,

  • Created: 21 July 2017
  • 2 MB
  • PDF
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Seahorse XF Mito Fuel Flex Test Kit

Measure the use of glucose, glutamine, and fatty acids as mitochondrial fuels in live cells

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The Agilent Seahorse Mito Fuel Flex Test kit (also known as the XF Fuel Inhibitor Pack) provides an analytical method for measuring mitochondrial fuel oxidation in live cells. A set of inhibitors are used to reveal the cells’ ability to switch oxidative pathways to meet energetic demands. It provides information on the relative contributions of glucose, glutamine, and long-chain fatty acid oxidation.|Note: The name of this product will be changed by October 2024. The same components will be available under the new product name “XF Fuel Inhibitor Pack”.
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Seahorse XF Plasma Membrane Permeabilizer

A detergent-free cell permeabilization reagent

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The Agilent Seahorse XF Plasma Membrane Permeabilizer (PMP) is a proprietary reagent that permeabilizes intact cells in culture. It selectively targets the cellular plasma membrane while leaving the mitochondrial membrane intact. This selectivity enables the detailed characterization of key components in mitochondrial function including transporters, enzymes, and electron transport chain complexes.
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Seahorse XF Real-Time ATP Rate Assay Kit

Quantify the rate of ATP production from glycolysis and mitochondrial respiration simultaneously

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The Agilent Seahorse ATP Real-Time rate assay is the only assay that quantifies the rate of adenosine triphosphate (ATP) production from glycolysis and mitochondria simultaneously using label-free technology in live cells. The real-time kinetic quantification delivers a dynamic picture of cellular bioenergetics, providing researchers with unique insights into cellular phenotype and function. Each kit contains the material sufficient for six full plate tests. The XF kit is for use with 96- or 24-well Seahorse Analyzers. The XFp kit is for use with 8-well Seahorse Analyzers.
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103010-100

Seahorse XFp Cell Mito Stress Test Kit. Each kit contains six (6) single-use pouches. Each pouch contains one (1) each of oligomycin, FCCP, and rotenone/antimycin A. For use with Seahorse XF HS Mini and XFp analyzers.

  • Unit:

    1 Kit

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