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Technical Overviews

UltrAA lamps — Increase sensitivity by up to 40% (PDF)

Agilent UltrAA hollow cathode lamps lower detection limits for the most demanding flame, furnace and vapor AA applications.

Publication Part Number: 5990-6711EN
Created: 23 Apr 2013
1 MB
PDF

Authors	Field NS, Elbulok OA, Dybas JM, Moser EK, Dar AA, Spruce LA, Fazelinia H, Seeholzer SH and Oliver PM
Journal	Eur J Immunol
Publication Date	01 Oct 2020
Research Area(s) include	Immunology Research
Technique	Seahorse XF Technology
Analyzer(s) include	Seahorse XF 96 well (XF Pro/XFe96/XF96), Seahorse XFe24/XF24, Seahorse XFp
Mode	N/A
Cell Line(s) include	T-cells
Cell Type(s) include	Immune cells
Species	Mouse
Assay / Method	Cell Mitochondrial Stress Test, Glycolysis Stress Test
Cell Seeding Density	2.0x10^5 cells/well
Plate Coating	Cell-Tak

Authors	Dematteis G, Vydmantaite G, Ruffinatti FA, Chahin M, Farruggio S, Barberis E, Ferrari E, Marengo E, Distasi C, Morkuniene R, Genazzani AA, Grilli M, Grossini E, Corazzari M, Manfredi M, Lim D, Jekabsone A and Tapella L
Journal	Cell Death Dis
Publication Date	18 Aug 2020
Research Area(s) include	Neurobiology Research
Technique	Seahorse XF Technology
Analyzer(s) include	Seahorse XFp
Mode	N/A
Cell Line(s) include	Astrocytes
Cell Type(s) include	Neuronal cells
Species	Mouse
Assay / Method	Cell Mitochondrial Stress Test, Real-Time ATP Rate Assay
Cell Seeding Density	1.0x10^5 cells/well
Plate Coating	Not Specified

Authors	Zhang, X, Wang, R, Hu, D, Sun, X, Fujioka, H, Lundberg, K, Chan, ER, Wang, Q, Xu, R, Flanagan, ME, Pieper, AA and Qi, X
Journal	Sci Adv
Publication Date	06 Dec 2020
Research Area(s) include	Immunology Research, Neurobiology Research
Technique	Seahorse XF Technology
Analyzer(s) include	Seahorse XFp
Mode	N/A
Cell Line(s) include	Oligodendrocyte
Cell Type(s) include	Neuronal cells
Species	Human
Assay / Method	Cell Mitochondrial Stress Test, Glycolysis Stress Test
Cell Seeding Density	Not Specified
Plate Coating	Not Specified

Authors	Gupta A, Beg M, Kumar D, Shankar K, Varshney S, Rajan S, Srivastava A, Singh K, Sonkar S, Mahdi AA, Dikshit M, Gaikwad AN
Journal	Free Radic Biol Med
Publication Date	22 July 2017
Research Area(s) include	Obesity, Diabetes, and Metabolic Disorders
Technique	Seahorse XF Technology
Analyzer(s) include	Seahorse XFp
Mode	N/A
Cell Line(s) include	3T3-L1
Cell Type(s) include	Preadipocytes
Species	Mouse
Assay / Method	Cell Mitochondrial Stress Test
Cell Seeding Density	Not Specified
Plate Coating	Not Specified

Authors	VanLinden MR, Dölle C, Pettersen IK, Kulikova VA, Niere M, Agrimi G, Dyrstad SE, Palmieri F, Nikiforov AA, Tronstad KJ, Ziegler M
Journal	J Biol Chem
Publication Date	01 Nov 2015
Research Area(s) include	Cell Physiology Research
Technique	Seahorse XF Technology
Analyzer(s) include	Seahorse XF 96 well (XF Pro/XFe96/XF96)
Cell Line(s) include	HEK293
Cell Type(s) include	Embryonic kidney cells
Species	Human
Assay / Method	Cell Mitochondrial Stress Test, Glycolysis Stress Test
Cell Seeding Density	3.0x10^4 cells/well
Plate Coating	Poly-L-lysine

Authors	Yang L, Jing Y, Wang W, Ying W, Lin L, Chang J, Luo L, Kang D, Jiang P, Liu J, Chen Q, Miller H, Herrada AA, Kubo M, Sun J and Liu C
Journal	Biochemical and Biophysical Research Communications
Publication Date	20 Aug 2020
Research Area(s) include	Immunology Research
Technique	Seahorse XF Technology
Analyzer(s) include	Seahorse XFe24/XF24
Cell Line(s) include	B-cells
Cell Type(s) include	Immune cells
Species	Mouse
Assay / Method	Cell Mitochondrial Stress Test
Cell Seeding Density	Not Specified
Plate Coating	Not Specified

Authors	Ramstead AG, Wallace JA, Lee SH, Bauer KM, Tang WW, Ekiz HA, Lane TE, Cluntun AA, Bettini ML, Round JL, Rutter J and O'Connell RM
Journal	Cell Rep
Publication Date	03 Mar 2020
Research Area(s) include	Immunology Research
Technique	Seahorse XF Technology
Analyzer(s) include	Seahorse XF 96 well (XF Pro/XFe96/XF96)
Cell Line(s) include	T-cells, Thymocytes
Cell Type(s) include	Immune cells
Species	Mouse
Assay / Method	Cell Mitochondrial Stress Test, Glycolysis Stress Test
Cell Seeding Density	1.3-1.8x10^6 cells/well, 3.6x10^6 cells/well
Plate Coating	Not Specified

Authors	Luongo TS, Lambert JP, Gross P, Nwokedi M, Lombardi AA, Shanmughapriya S, Carpenter AC, Kolmetzky D, Gao E, van Berlo JH, Tsai EJ, Molkentin JD, Chen X, Madesh M, Houser SR, Elrod JW
Journal	Nature
Publication Date	04 May 2017
Research Area(s) include	Cardiovascular Research, Translational Research
Technique	Seahorse XF Technology
Analyzer(s) include	Seahorse XF 96 well (XF Pro/XFe96/XF96)
Cell Line(s) include	Cardiomyocytes
Cell Type(s) include	Cardiac cells
Species	Human
Assay / Method	Cell Mitochondrial Stress Test, Substrate Utilization
Cell Seeding Density	2.5x10^4 cells/well
Plate Coating	Not Specified

Authors	Ni Y, Hagras MA, Konstantopoulou V, Mayr JA, Stuchebrukhov AA, Meierhofer D
Journal	Cells
Publication Date	25 Sep 2019
Research Area(s) include	Mitochondrial Disease Research
Technique	Seahorse XF Technology
Analyzer(s) include	Seahorse XF 96 well (XF Pro/XFe96/XF96)
Cell Line(s) include	Dermal Fibroblasts
Cell Type(s) include	Fibroblast
Species	Human
Assay / Method	Cell Mitochondrial Stress Test, Glycolytic Rate Assay
Cell Seeding Density	4.0x10^4 cells/well
Plate Coating	Not Specified

Authors	Hornig-Do HT, Montanari A, Rozanska A, Tuppen HA, Almalki AA, Abg-Kamaludin DP, Frontali L, Francisci S, Lightowlers RN, Chrzanowska-Lightowlers ZM
Journal	EMBO Mol Med
Publication Date	01 Jan 2014
Research Area(s) include	Mitochondrial Disease Research
Technique	Seahorse XF Technology
Analyzer(s) include	Seahorse XFe24/XF24
Cell Line(s) include	143B.206 rho0
Cell Type(s) include	Osteosarcoma cells
Species	Human
Assay / Method	Cell Mitochondrial Stress Test
Cell Seeding Density	1.0x10^4 cells/well
Plate Coating	Not Specified

Authors	Carnevale J, Shifrut E, Kale N, Nyberg WA, Blaeschke F, Chen YY, Li Z, Bapat SP, Diolaiti ME, O'Leary P, Vedova S, Belk J, Daniel B, Roth TL, Bachl S, Anido AA, Prinzing B, Ibanez-Vega J, Lange S, Haydar D, Luetke-Eversloh M, Born-Bony M, Hegde B, Kogan S, Feuchtinger T, Okada H, Satpathy AT, Shannon K, Gottschalk S, Eyquem J, Krenciute G, Ashworth A and Marson A
Journal	Nature
Publication Date	01 Sep 2022
Research Area(s) include	Cancer Research, Immunology Research
Technique	Seahorse XF Technology
Analyzer(s) include	Seahorse XF 96 well (XF Pro/XFe96/XF96)
Mode	N/A
Cell Line(s) include	CAR-T cells, T-cells
Cell Type(s) include	Immune cells
Species	Human
Assay / Method	Cell Mitochondrial Stress Test, Substrate Oxidation Stress Test
Cell Seeding Density	4.0x10^5 cells/well
Plate Coating	Poly-D-lysine

Authors	Bajpai R, Sharma A, Achreja A, Edgar CL, Wei C, Siddiqa AA, Gupta VA, Matulis SM, McBrayer SK, Mittal A, Rupji M, Barwick BG, Lonial S, Nooka AK, Boise LH, Nagrath D and Shanmugam M
Journal	Nat Commun
Publication Date	06 Mar 2020
Research Area(s) include	Cancer Research
Technique	Seahorse XF Technology
Analyzer(s) include	Seahorse XF 96 well (XF Pro/XFe96/XF96)
Cell Line(s) include	Myelomonocytic
Cell Type(s) include	Leukemia cells
Species	Human
Assay / Method	Cell Mitochondrial Stress Test
Cell Seeding Density	8.0x10^4 cells/well
Plate Coating	Cell-Tak

Authors	Chen C-y, Li Y, Jia T, He L, Hare AA, Silberstein A, Gallagher J, Martinez TF, Stiles JW, Olenyuk B, Dervan PB and Stiles BL
Journal	Medicinal Chemistry Research
Publication Date	26 Feb 2020
Research Area(s) include	Cancer Research
Technique	Seahorse XF Technology
Analyzer(s) include	Seahorse XFe24/XF24
Cell Line(s) include	Hepatocytes
Cell Type(s) include	Hepatocytes
Species	Human, Mouse
Assay / Method	Cell Mitochondrial Stress Test
Cell Seeding Density	2.0x10^4 cells/well, 4.0x10^4 cells/well
Plate Coating	Not Specified

Authors	Heinsbroek SE, Squadrito ML, Schilderink R, Hilbers FW, Verseijden C, Hofmann M, Helmke A, Boon L, Wildenberg ME, Roelofs JJ, Ponsioen CY, Peters CP, Te Velde AA, Gordon S, De Palma M, de Jonge WJ
Journal	Mucosal Immunol
Publication Date	01 Nov 2015
Research Area(s) include	Immunology Research
Technique	Seahorse XF Technology
Analyzer(s) include	Seahorse XF 96 well (XF Pro/XFe96/XF96)
Cell Line(s) include	Bone marrow-derived macrophages (BMDM)
Cell Type(s) include	Immune cells
Species	Mouse
Assay / Method	Cell Mitochondrial Stress Test
Cell Seeding Density	Not Specified
Plate Coating	Not Specified

Authors	Jadiya P, Kolmetzky DW, Tomar D, Di Meco A, Lombardi AA, Lambert JP, Luongo TS, Ludtmann MH, Pratico D, and Elrod JW
Journal	Nat Commun
Publication Date	29 Aug 2019
Research Area(s) include	Neurobiology Research
Technique	Seahorse XF Technology
Analyzer(s) include	Seahorse XF 96 well (XF Pro/XFe96/XF96)
Cell Line(s) include	Cerebral cortical neurons
Cell Type(s) include	Neuronal cells
Species	Mouse
Assay / Method	Cell Mitochondrial Stress Test
Cell Seeding Density	3.0x10^4 cells/well
Plate Coating	Not Specified

Authors	Birket MJ, Orr AL, Gerencser AA, Madden DT, Vitelli C, Swistowski A, Brand MD, Zeng X
Journal	J Cell Sci
Publication Date	01 Feb 2011
Research Area(s) include	Cell Physiology Research
Technique	Seahorse XF Technology
Analyzer(s) include	Seahorse XFe24/XF24
Cell Line(s) include	Embryonic stem cells (ESC), Fibroblasts, Neural stem cells
Cell Type(s) include	Fibroblast, Stem cells
Species	Human
Assay / Method	Cell Mitochondrial Stress Test
Cell Seeding Density	3.0x10^4 cells/well, 7.0x10^4 cells/well, Not Specified
Plate Coating	Geltrex, Not Specified

Authors	Bustos RI, Jensen EL, Ruiz LM, Rivera S, Ruiz S, Simon F, Riedel C, Ferrick D, Elorza AA
Journal	Biochem Biophys Res Commun
Publication Date	01 Aug 2013
Research Area(s) include	Cell Physiology Research
Technique	Seahorse XF Technology
Analyzer(s) include	Seahorse XFe24/XF24
Cell Line(s) include	K562
Cell Type(s) include	Leukemia cells
Species	Human
Assay / Method	Cell Mitochondrial Stress Test
Cell Seeding Density	8.0x10^4 cells/well
Plate Coating	Poly-lysine

Authors	Liu M, Tong Z, Ding C, Luo F, Wu S, Wu C, Albeituni S, He L, Hu X, Tieri D, Rouchka EC, Hamada M, Takahashi S, Gibb AA, Kloecker G, Zhang HG, Bousamra M, 2nd, Hill BG, Zhang X and Yan J
Journal	J Clin Invest
Publication Date	01 Apr 2020
Research Area(s) include	Cancer Research, Immunology Research
Technique	Seahorse XF Technology
Analyzer(s) include	Seahorse XFe24/XF24
Cell Line(s) include	Bone marrow-derived macrophages (BMDM)
Cell Type(s) include	Immune cells
Species	Mouse
Assay / Method	Cell Mitochondrial Stress Test with additional injection
Cell Seeding Density	6.0x10^4 cells/well
Plate Coating	Not Specified

Authors	Zhou G, Wang J, Zhao M, Xie TX, Tanaka N, Sano D, Patel AA, Ward AM, Sandulache VC, Jasser SA, Skinner HD, Fitzgerald AL, Osman AA, Wei Y, Xia X, Songyang Z, Mills GB, Hung MC, Caulin C, Liang J, Myers JN
Journal	Mol Cell
Publication Date	01 June 2014
Research Area(s) include	Cancer Research
Technique	Seahorse XF Technology
Analyzer(s) include	Seahorse XFe24/XF24
Cell Line(s) include	Detroit 562
Cell Type(s) include	Squamous Cell Carcinoma
Species	Human
Assay / Method	Cell Mitochondrial Stress Test with additional injection
Cell Seeding Density	Not Specified
Plate Coating	Not Specified