Full characterization of a particular protein of interest can require in-depth, accurate analysis of both the intact and digested forms.For cost effective and rapid molecular weight determination Agilent offers the microfluidic based Bioanalyzer 2100 for turnkey sizing, quantification and quality control of proteins. The High mass accuracy of the 6550 Q-TOF MS combined with the high sensitivity allows highly accurate protein molecular weight confirmation and elucidation of low level minor components. MassHunter Bioconfirm software enables very high-definition comparison between protein components, and is especially valuable when looking for minor protein isoform changes. Bioconfirm software also makes it easy to correlate peptide mapping data with extremely accurate intact molecular weight measurement. The mRP column technology provides outstanding capabilities to recover >98% of proteins and peptides, desalt, concentrate, and fractionate samples in one easy step. The 3100 OFFGEL system enables pre-fractionation of proteins and peptides prior to LC/MS analysis to increase the number of proteins identified.