Cell Physiology
Evaluating Changes in Cell Physiology
Cellular function, particularly in regard to cell metabolism, is still not fully understood. Agilent Cell Analysis technology and assays provide new understanding of cell physiology research, by measuring cell metabolism in real time. These capabilities give researchers the most physiologically relevant data to further their scientific research into cell physiology.
Learn more about How Seahorse XF Technology Works
Learn more about MitoXpress & pH-Xtra Products
Click to search either the Agilent Cell Analysis Publications Database or the Cell Reference Database
For Research Use Only. Not for use in diagnostic procedures.
Measuring glycolysis and oxidative metabolism in cancer cells
- PDF/ Found In: Application Notes
- Date : 07 Mar 2017
- File Size : 721.04 KB
Assessing the impact of autophagy on cellular metabolism
- PDF/ Found In: Application Notes
- Date : 22 Dec 2016
- File Size : 1.06 MB
- Application Notes
-
Analysis of Phosphorylated STAT Protein Signaling in Lymphocytes Using Flow Cytometry
Examines the phosphorylation of signal transducer and activator of transcription (STAT) proteins in response to cytokine stimulation using a phosphoflow technique.
- Application Notes
- English
- 27 Nov 2019
- 1.13 MB
Detection of Small Particles by Flow Cytometry: Analysis of Small Particle Beads and Extracellular Vesicles
Describes the ability of the Agilent NovoCyte Quanteon flow cytometer to resolve small particles, including cell-derived extracellular vesicles (EV) and beads.
- Application Notes
- English
- 05 Nov 2019
- 1.50 MB
Monitoring of Mammalian Cell Culture Media with HILIC-LC/MS
Quantitation and tracking of metabolites in a mammalian cell culture using HILIC with LC/MS detection
- Application Notes
- English
- 15 Apr 2019
- 1.19 MB
Determination of antioxidant capacity in human serum using the Agilent Cary Eclipse for the ORAC assay
The Agilent Cary Eclipse fluorescence spectrophotometer with Peltier-thermostatted multicell holder was used to perform the ORAC (oxygen radical absorbance capacity) assay to
- Application Notes
- English
- 13 Feb 2018
- 282.81 KB
Measuring glycolysis and oxidative metabolism in cancer cells
Real-time assay enables scientists to connect metabolic pathways with oncogenic phenotypes
- Application Notes
- English
- 07 Mar 2017
- 721.04 KB
Assessing functional metabolism using cells sourced from blood
Real-time, kinetic measurements of functional metabolism in cells sourced from blood link mitochondrial function to disease diagnosis and progression.
- Application Notes
- English
- 04 Jan 2017
- 643.12 KB
Identifying Metabolic Switches Using an Agilent Seahorse XFe Analyzer in Hypoxia
Identifying Metabolic Phenotype Switches in Cancer Cells Using the Agilent Seahorse XF/XFe Analyzer in an Hypoxic Environment
- Application Notes
- English
- 03 Jan 2017
- 744.28 KB
Measure Mitochondrial Respiration in Skeletal Muscle Fibers with a Seahorse Analyzer
Measuring Mitochondrial Respiration in Intact Skeletal Muscle Fibers with an Agilent Seahorse XF24/XFe24 Analyzer
- Application Notes
- English
- 22 Dec 2016
- 1.07 MB
Bioenergetic analysis of suspension cells: hematopoietic stem cells and lymphocytes
A real-time assay that quantifies the ATP and biosynthetic demands of immune cell proliferation, differentiation and effector function.
- Application Notes
- English
- 22 Dec 2016
- 634.88 KB
Assessing the impact of autophagy on cellular metabolism
Real-time, functional metabolic assays connect energy pathways and autophagy
- Application Notes
- English
- 22 Dec 2016
- 1.06 MB
同时测量外源性和内源性脂肪酸的氧化情况
代谢通路和能量消耗的调节,以及底物利用如何促进细胞信号传导已成为代谢和心血管生理学、免疫学和癌症生物学的新兴领域。值得特别关注的是,脂肪酸的氧化,内源性地储存在细胞内并且可外源性地获得。XF 棕榈酸盐-BSA FAO 底物和 Seahorse XFe 细胞外流量分析仪可提供动力学、非破坏性的方法来研究粘附细胞单层内的脂肪酸氧化。
XF 生物能量代谢检测漫游指南
XF 体外代谢流技术通常用于测量细胞能量代谢,以及检测代谢在调节细胞表型中的作用。从测量基础胞内线粒体功能起,现在 XF 分析发展为能够应用多种检测测量线粒体底物选择性和糖酵解。此外,XF 分析仪还可用于测量分离线粒体的功能,可同时检查多个样品的线粒体,节省宝贵的时间和资源。
能量通路视频
- 00:02:06
测定细胞代谢 — 瞬态微室视频
- 00:02:12