Cardiovascular Research
Exploring Mitochondrial Function and Cardiovascular Research
The cardiovascular system has a constant requirement for optimal energy to fuel its function. Mitochondria not only generates adenosine triphosphate (ATP), the cellular energy currency, but regulates cell function. Mitochondrial dysfunction, in addition to increased oxidative stress, has been linked to cardiovascular diseases. Agilent Cell Analysis technology is an integral part of a cardiovascular research strategy, enabling researchers to uncover novel insights into cellular mechanisms.
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Click to search either the Agilent Cell Analysis Publications Database or the Cell Reference Database
For Research Use Only. Not for use in diagnostic procedures.
Assessing functional metabolism using cells sourced from blood
- PDF/ Found In: Application Notes
- Date : 04 Jan 2017
- File Size : 643.12 KB
Measuring glycolytic function in cells
- PDF/ Found In: Application Notes
- Date : 08 Mar 2017
- File Size : 1.07 MB
- Application Notes
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Analysis of Phosphorylated STAT Protein Signaling in Lymphocytes Using Flow Cytometry
Examines the phosphorylation of signal transducer and activator of transcription (STAT) proteins in response to cytokine stimulation using a phosphoflow technique.
- Application Notes
- English
- 27 Nov 2019
- 1.13 MB
Measuring glycolytic function in cells
Real-time, kinetic measurement of glycolysis connects energy pathways to cell growth and proliferation
- Application Notes
- English
- 08 Mar 2017
- 1.07 MB
Identifying Metabolic Switches Using an Agilent Seahorse XFe Analyzer in Hypoxia
Identifying Metabolic Phenotype Switches in Cancer Cells Using the Agilent Seahorse XF/XFe Analyzer in an Hypoxic Environment
- Application Notes
- English
- 03 Jan 2017
- 744.28 KB
Measuring Fatty Acid Oxidation with the Seahorse XF Analyzer of Exogenous and Endogenous Fatty Acids Using the XF Palmitate-BSA FAO Substrate with the Agilent Seahorse XF Cell Mito Stress Test
Measuring Oxidation of Exogenous and Endogenous Fatty Acids Using the XF Palmitate-BSA FAO Substrate with the Agilent Seahorse XF Cell Mito Stress Test
- Application Notes
- English
- 22 Dec 2016
- 1.13 MB
UHPLC-MS/MS Triple Quadrupole Analysis of Anthocyanin Metabolites in Human Plasma
Anthocyanins are studied in human dosing of strawberry drink by LC/QQQ at low levels in plasma. SPE is to be superior for sample prep and conditions are given.
- Application Notes
- English
- 04 Mar 2016
- 303.27 KB
利用 CRISPR/Cas9 技术改变干细胞基因组SNP进行单核苷酸多态性分析,以研究心血管疾病
在本次网络研讨会中,Chris Denning 教授将介绍与他研究相关的一些小故事,特别是关于使用 Cas9/CRISPR 对 hiPSC-CM 基因组进行修改,以及应用安捷伦 Seahorse XF 和 CardioECR 更深入了解人类疾病和变异的相关信息。人多能干细胞 (hPSC-CMs) 衍生的心肌细胞为建模分析疾病和药物对结构和功能的影响提供了强大的工具。本次报告还将提供使用 Cas9/CRISPR 报告细胞系对可能有利于诱导心肌细胞成熟的化合物进行大规模筛选的早期数据。
细胞在生理条件下和应激反应时的线粒体能量代谢谱
一种非常有用且直接的方法是依次添加 ATP 合成酶(寡霉素)抑制剂、解联剂 (FCCP) 和呼吸链抑制剂(抗霉素或鱼藤酮),以便在基础和压力条件下获得细胞的线粒体特性。
暴露于氧化应激条件下的心血管细胞中线粒体储备能力的重要性。
活性氧类物质和氮类物质是正常代谢的一部分,在心血管(病理)生理学中起着重要作用。有趣的是,这些活性物质或它们的次级产物能够共价修饰蛋白质,从而调节酶功能或引发氧化还原信号转导。特别是线粒体,它是这些活性物质的主要来源和氧化蛋白修饰的热点。
能量通路视频
- 00:02:06
测定细胞代谢 — 瞬态微室视频
- 00:02:12