GKE-5016D
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Endoglycosidases

Part Number:
GKE-5016D

AdvanceBio N-Glycanase (PNGase F, EDTA free), ≥2.5 U/mL (formerly ProZyme). A recombinant form of PNGase F (1U, EDTA free), releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. Does not include reaction buffers that ship with 'A' (100 mU) and 'B' (200 mU) pack sizes. Please contact us if you require buffers.

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List Price:
$1,996.00

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Specifications
Concentration
  • 2.5 U/mL
Enzyme Formulation
  • 20 mM Tris-HCl, 50 mM NaCl (pH 7.5)
Enzyme Source
  • Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as Endoglycosidase F2, endo-beta-N-acetylglucosaminidase. Enzyme also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase.
Enzyme Specific Activity
  • ≥10 units/mg
Enzyme Specificity
  • Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless a(1-3) core fucosylated, as in plant glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate, and sialic acid groups attached to the oligosaccharide do not affect cleavage. Endo F free. Highly concentrated, particularly useful for deglycosylation under native conditions.
Enzyme Unit Definition
  • One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 µmole of denatured ribonuclease B per minute at 37°C, pH 7.5.
Molecular Weight
  • ~35,000 Daltons
Unit
  • 1 U
Volume
  • 400 µL
pH Optimum
  • 8.6
pH Range
  • 7.5-9.5
Support
Safety Data Sheets

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

  • Safety Data Sheets
  • English
  • 12-May-2019
  • 171.15 Kb
  • PDF

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

  • Safety Data Sheets
  • English
  • 12-May-2019
  • 168.82 Kb
  • PDF

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

  • Safety Data Sheets
  • English
  • 12-May-2019
  • 199.62 Kb
  • PDF

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

  • Safety Data Sheets
  • English
  • 12-May-2019
  • 69.07 Kb
  • PDF

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