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AdvanceBio Gly-X Technology

AdvanceBio Gly-X N-glycan prep with InstantAB kit, 96-ct (formerly ProZyme) utilizes a novel in-solution enzymatic protein deglycosylation followed by rapid instant labeling of released N-glycans with InstantAB dye. With deglycosylation and labeling carried out in solution, the method is simple, rapid, and suitable for automation. After a simple cleanup step, the InstantAB-labeled glycan samples are ready for analysis by UHPLC, LC/MS, and other methods. Includes GX96-100 Gly-X deglycosylation module, GX96-501 Gly-X InstantAB labeling module, GX96-502 Gly-X InstantAB cleanup module.

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  • Instant labeling with InstantAB
  • 5-minute N-Glycanase (PNGase F) digestion provides unbiased N-glycan release
  • Flexible, high-throughput format: process 1 to 96 samples
  • Optimized cleanup removes excess free dye, protein, and other interfering compounds.
Kit Configuration
  • Sample Prep Kit
  • 96
  • LC
Prep time (approx)
  • ~1 hour
Equipment and Reagents Provided by User
  • 96-well Thermocycler or two independent heat blocks, set for 90°C and 50°C
  • Thermocycler (Corning THERM-1001, 110V. THERM-1000, 230V). Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating Blocks
  • Vacuum manifold (Millipore MSVMHTS00-HTS)
  • Gly-X vacuum manifold spacer (GX100)
  • Vacuum pump (Millipore WP6211560, 110 V. WP6122050, 220V. Welch WOB-L Pump 2522)
  • Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended)