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AdvanceBio Gly-X Technology

AdvanceBio Gly-X N-glycan prep with APTS Express kit, 96-ct (formerly ProZyme) utilizes a novel in-solution enzymatic protein deglycosylation followed by Express labeling of released N-glycans with 8-aminopyrene-1,3,6-trisulfonic acid (APTS). With deglycosylation carried out in solution, the method is simple, rapid and suitable for automation. After a simple cleanup step, the APTS-labeled glycan samples are ready for analysis by CE, CE/MS, and other methods. Includes GX96-100 Gly-X deglycosylation module (96-ct), GX96-601 Gly-X APTS Express labeling module (96-ct), GX96-602 Gly-X APTS Express cleanup module (96-ct).

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  • Reductive amination APTS Express labeling chemistry without dry down
  • 5-minute N-Glycanase (PNGase F) digestion provides unbiased N-glycan release
  • Flexible, high-throughput format: process 1 to 96 samples
  • Optimized cleanup removes excess free dye, protein, and other interfering compounds.
Kit Configuration
  • Sample Prep Kit
  • 96
Protein Load Range
  • 1-40 µg
  • CE
Prep time (approx)
  • ~2.5 hours
Equipment and Reagents Provided by User
  • 96-well Thermocycler or two independent heat blocks, set for 90°C and 50°C
  • Thermocycler (Corning THERM-1001, 110 V. THERM-1000, 230 V)
  • Vacuum manifold (Millipore MSVMHTS00-HTS)
  • Vacuum pump (Millipore WP6211560, 110 V. WP6122050, 220V. Welch WOB-L pump 2522)
  • Vacuum manifold spacer (GX100, or GX400 Gly-X 2-AB/APTS starter kit is included free of charge with first Gly-X order, please contact us for details)
  • Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended
  • DI Water