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AdvanceBio Gly-X Technology

AdvanceBio Gly-X 2-AB Express labeling module, 96-ct (formerly ProZyme) utilizes 2-AB (2-aminobenzamide) dye to derivatize released glycans. The proven 2-AB dye assures data continuity for ongoing projects.

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  • Reductive amination 2-AB labeling chemistry without dry down
  • 5-minute N-Glycanase (PNGase F) digestion provides unbiased N-glycan release
  • Flexible, high-throughput format: process 1 to 96 samples
  • Optimized cleanup removes excess free dye, protein, and other interfering compounds.
Kit Configuration
  • Sample Prep Module
  • 96
  • 80 %
Protein Load Range
  • 1-40 µg
  • LC & LC/MS
Deglycosylation Efficiency
  • 99 %
Prep time (approx)
  • 130 min (24 samples)
  • At least 1 month at -20°C, 5 days at 4–8°C
Equipment and Reagents Provided by User
  • Two dry block heaters with 96 well PCR heat blocks. One of the heaters can be substituted with a 96-well thermocycler. Denaturation and deglycosylation can be performed using a thermocycler. A heat block is required for the labeling step.
  • Two Thermo Scientific 88871001 Compact dry block heaters with 96 well PCR heat blocks.
  • Gly-X vacuum manifold spacer and heater block lid (included in GX400 Gly-X 2-AB Express starter kit, or use GX100 spacer and alternate heater lid)
  • Vacuum manifold (Millipore MSVMHTS00)
  • Vacuum pump (Millipore WP6211560, 110 V. WP6122050, 220 V. Welch WOB-L Pump 2522)
  • DI water, ~100 µl per sample
  • Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended), ~4 mL per sample
Preservation of Sialylated Species
  • 99%