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Part Number:
GX96-2AB

AdvanceBio Gly-X Technology

AdvanceBio Gly-X N-glycan prep with 2-AB Express kit, 96-ct (formerly ProZyme). Utilizes a novel in-solution enzymatic protein deglycosylation followed by Express labeling of released N-glycans with 2-AB dye. With deglycosylation carried out in solution, the method is simple, rapid, and suitable for automation. After a simple cleanup step, the 2-AB-labeled glycan samples are ready for analysis by UHPLC, LC/MS, MS/MS and other methods. Includes GX96-100 Gly-X deglycosylation module (96-ct), GX96-401 Gly-X 2-AB Express labeling module (96-ct), GX96-402 Gly-X 2-AB Express cleanup module (96-ct).

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Specifications
Application
  • Reductive amination 2-AB labeling chemistry without dry down
  • 5-minute N-Glycanase (PNGase F) digestion provides unbiased N-glycan release
  • Flexible, high-throughput format: process 1 to 96 samples
  • Optimized cleanup removes excess free dye, protein, and other interfering compounds.
Kit Configuration
  • Sample Prep Kit
Count
  • 96
Recovery
  • 80 %
UNSPSC Code
  • 41115711
Protein Load Range
  • 1-40 µg
Technique
  • LC & LC/MS
Deglycosylation Efficiency
  • 99 %
Prep time (approx)
  • ~2.5 hours
Stability
  • At least 1 month at -20°C, 5 days at 4–8°C
Equipment and Reagents Provided by User
  • 96-well Thermocycler or two independent heat blocks
  • Thermocycler (Corning THERM-1001, 110V, THERM-1000, 230V)
  • Vacuum manifold (Millipore MSVMHTS00-HTS)
  • Vacuum pump (Millipore WP6211560, 110 V, WP6122050, 220V, Welch WOB-L Pump 2522)
  • Vacuum manifold spacer (GX100, or GX400 Gly-X 2-AB starter kit - included free of charge with first Gly-X order, please contact us for details)
  • Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended)
  • DI water
Preservation of Sialylated Species
  • 99%