Please login to add product to your favorites.

AdvanceBio Gly-X Technology

Part Number:
GX96-202IAB

AdvanceBio Gly-X N-glycan preparation and labeling with InstantAB module set, 96-ct (formerly ProZyme), utilizes a novel in-solution enzymatic protein deglycosylation followed by rapid instant labeling of released N-glycans with InstantAB dye. With deglycosylation and labeling carried out in solution, the method is simple, rapid, and suitable for automation. After a simple cleanup step, the InstantAB-labeled glycan samples are ready for analysis by UHPLC, LC/MS, MS/MS and other methods. Includes GX96-100 Gly-X deglycosylation module, GX96-501 Gly-X InstantAB labeling module.

Item successfully added to your list
Request Quote

Please enter valid quantity

Specifications
Application
  • Instant labeling with InstantAB:5-minute N-Glycanase (PNGase F) digestion provides unbiased N-glycan release
  • Flexible, high-throughput format: process 1 to 96 samples
Kit Configuration
  • Sample Prep Module Set
Count
  • 96
Protein Load Range
  • 1-40 µg
Technique
  • LC
Prep time (approx)
  • ~1 hour
Stability
  • Samples may be stored at -20°C for at least 6 months or 4°C for up to 5 days.
Equipment and Reagents Provided by User
  • 96-well Thermocycler or two independent heat blocks, set for 90°C and 50°C. (e.g. Corning THERM-1001, 110V. THERM-1000, 230V). Note: Two GlykoPrep heaters, GS150, can be fitted with VWR 13259-260 modular heating blocks.
  • Vacuum manifold (Millipore MSVMHTS00)
  • Vacuum pump (Millipore WP6211560, 110 V, WP6122050, 220V, Welch WOB-L Pump 2522). Note: If you have a vacuum manifold other than the Millipore model suggested, contact us for setup instructions
  • Gly-X Vacuum manifold spacer (ProZyme GX100)
  • Formic acid, MS-grade (Fisher A117-50 recommended)
  • Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended)
  • Optional trifluoroacetic acid (TFA)
  • Optional VWR 10 kDa centrifugal filters (82031-348)
Resources

AdvanceBio Gly-X Deglycosylation and InstantAB Labeling Module set, 96-ct

Gly-X-Glycanase, 1 mg/mL, 120 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, Gly-X InstantAB Dye Solvent, 18.5mg, Gly-X InstantAB Dye Solvent, 375μl

AdvanceBio Gly-X Deglycosylation and InstantAB Labeling Module set, 96-ct

Gly-X-Glycanase, 1 mg/mL, 120 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, Gly-X InstantAB Dye Solvent, 18.5mg, Gly-X InstantAB Dye Solvent, 375μl

AdvanceBio Gly-X Deglycosylation and InstantAB Labeling Module set, 96-ct

Gly-X-Glycanase, 1 mg/mL, 120 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, Gly-X InstantAB Dye Solvent, 18.5mg, Gly-X InstantAB Dye Solvent, 375μl

AdvanceBio Gly-X Deglycosylation and InstantAB Labeling Module set, 96-ct

Gly-X-Glycanase, 1 mg/mL, 120 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, Gly-X InstantAB Dye Solvent, 18.5mg, Gly-X InstantAB Dye Solvent, 375μl

AdvanceBio Gly-X Deglycosylation and InstantAB Labeling Module set, 96-ct

Gly-X-Glycanase, 1 mg/mL, 120 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, Gly-X InstantAB Dye Solvent, 18.5mg, Gly-X InstantAB Dye Solvent, 375μl

AdvanceBio Gly-X Deglycosylation and InstantAB Labeling Module set, 96-ct

Gly-X-Glycanase, 1 mg/mL, 120 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, Gly-X InstantAB Dye Solvent, 18.5mg, Gly-X InstantAB Dye Solvent, 375μl

AdvanceBio Gly-X Deglycosylation and InstantAB Labeling Module set, 96-ct

Gly-X-Glycanase, 1 mg/mL, 120 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, Gly-X InstantAB Dye Solvent, 18.5mg, Gly-X InstantAB Dye Solvent, 375μl

AdvanceBio Gly-X Deglycosylation and InstantAB Labeling Module set, 96-ct

Gly-X-Glycanase, 1 mg/mL, 120 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, Gly-X InstantAB Dye Solvent, 18.5mg, Gly-X InstantAB Dye Solvent, 375μl

AdvanceBio Gly-X Deglycosylation and InstantAB Labeling Module set, 96-ct

Gly-X-Glycanase, 1 mg/mL, 120 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, Gly-X InstantAB Dye Solvent, 18.5mg, Gly-X InstantAB Dye Solvent, 375μl

AdvanceBio Gly-X Deglycosylation and InstantAB Labeling Module set, 96-ct

Gly-X-Glycanase, 1 mg/mL, 120 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, Gly-X InstantAB Dye Solvent, 18.5mg, Gly-X InstantAB Dye Solvent, 375μl

AdvanceBio Gly-X Deglycosylation and InstantAB Labeling Module set, 96-ct

Gly-X-Glycanase, 1 mg/mL, 120 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, Gly-X InstantAB Dye Solvent, 18.5mg, Gly-X InstantAB Dye Solvent, 375μl

AdvanceBio Gly-X Deglycosylation and InstantAB Labeling Module set, 96-ct

Gly-X-Glycanase, 1 mg/mL, 120 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, Gly-X InstantAB Dye Solvent, 18.5mg, Gly-X InstantAB Dye Solvent, 375μl

AdvanceBio Gly-X Deglycosylation and InstantAB Labeling Module set, 96-ct

Gly-X-Glycanase, 1 mg/mL, 120 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, Gly-X InstantAB Dye Solvent, 18.5mg, Gly-X InstantAB Dye Solvent, 375μl

AdvanceBio Gly-X Deglycosylation and InstantAB Labeling Module set, 96-ct

Gly-X-Glycanase, 1 mg/mL, 120 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, Gly-X InstantAB Dye Solvent, 18.5mg, Gly-X InstantAB Dye Solvent, 375μl

AdvanceBio Gly-X Deglycosylation and InstantAB Labeling Module set, 96-ct

Gly-X-Glycanase, 1 mg/mL, 120 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, Gly-X InstantAB Dye Solvent, 18.5mg, Gly-X InstantAB Dye Solvent, 375μl

AdvanceBio Gly-X Deglycosylation and InstantAB Labeling Module set, 96-ct

Gly-X-Glycanase, 1 mg/mL, 120 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, Gly-X InstantAB Dye Solvent, 18.5mg, Gly-X InstantAB Dye Solvent, 375μl