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AdvanceBio Gly-X Technology

AdvanceBio Gly-X N-glycan preparation and labeling with InstantPC module set, 96-ct (formerly ProZyme)utilizes a novel in-solution enzymatic protein deglycosylation followed by rapid labeling of released N-glycans with InstantPC dye. With deglycosylation and labeling carried out in solution, the method is simple, rapid, and suitable for automation. The InstantPC dye delivers unmatched fluorescent brightness and MS signal, which enables a single labeling method to be deployed across different glycan analysis workflows. Includes GX96-100 Gly-X deglycosylation module, GX96-101 Gly-X InstantPC labeling module.

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  • Flexible, high-throughput format: process 1 to 24 samples
  • Small molecule size for improved chromatographic peak resolution
Kit Configuration
  • Sample Prep Module Set
  • 96
  • 95 %
Protein Load Range
  • 1-40 µg
  • LC & LC/MS
Prep time (approx)
  • 42 min (16 samples), 99 min (96 samples)
  • Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF
Equipment and Reagents Provided by User
  • 96-well Thermocycler or two independent heat blocks, set for 90 °C and 50 °C
  • Thermocycler (Corning THERM-1001, 110V. THERM-1000, 230V). Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks
Preservation of Sialylated Species
  • 95%