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AdvanceBio Gly-X Technology

AdvanceBio Gly-X InstantPC labeling module, 96-ct (formerly ProZyme) utilizes InstantPC dye to derivatize released glycans. The InstantPC dye delivers unmatched fluorescent brightness and MS signal, enabling a single labeling method to be deployed across different glycan analysis workflows. The small molecule size provides improved chromatographic peak resolution.

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  • 96
  • 95 %
  • Small molecule size for improved chromatographic peak resolution
Protein Load Range
  • 1-40 µg
  • LC & LC/MS
  • Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF
Equipment and Reagents Provided by User
  • 96-well Thermocycler or two independent heat blocks, set for 90°C and 50°C. (e.g. Corning THERM-1001, 110V. THERM-1000, 230V). Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks
  • Vacuum manifold (Millipore MSVMHTS00)
  • Vacuum pump (Millipore WP6211560, 110 V, WP6122050, 220V, Welch WOB-L Pump 2522). Note: If you have a vacuum manifold and pump other than the Millipore model suggested, please contact us for information. Alternative vacuum manifold may be used with a GX200 spacer in place of GX100 (see FAQs)
  • Gly-X vacuum manifold spacer (Agilent GX100)
  • Formic acid, MS-grade (Fisher A117-50 recommended)
  • Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended)
  • Optional - VWR 10 kDa centrifugal filters (82031-348)
Preservation of Sialylated Species
  • 95%