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Part Number:
GX24-401AB

AdvanceBio Gly-X Technology

AdvanceBio Gly-X with 2-AB Express deglycosylation and labeling module set, 24-ct (formerly ProZyme). Utilizes a novel in-solution enzymatic protein deglycosylation followed by Express labeling of released N-glycans with 2-AB dye. With deglycosylation carried out in solution, the method is simple, rapid and suitable for automation. Includes GX24-100 Gly-X deglycosylation module (24-ct), GX24-401 Gly-X 2-AB Express labeling module (24-ct).

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Specifications
Application
  • Reductive amination 2-AB labeling chemistry without dry down
  • 5-minute N-Glycanase (PNGase F) digestion provides unbiased N-glycan release
  • Flexible, high-throughput format: process 1 to 96 samples
Kit Configuration
  • Sample Prep Module Set
Count
  • 24
Recovery
  • 80 %
Protein Load Range
  • 1-40 µg
Technique
  • LC & LC/MS
Deglycosylation Efficiency
  • 99 %
Prep time (approx)
  • 130 min (24 samples)
Stability
  • At least 1 month at -20°C, 5 days at 4–8°C
Equipment and Reagents Provided by User
  • Gly-X 2-AB Express cleanup module (96-ct) [GX96-402]
  • 96-well Thermocycler or two independent heat blocks
  • Thermocycler (Corning THERM-1001, 110V
  • THERM-1000, 230V)
  • Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended)