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Part Number:
GX24-2AB

AdvanceBio Gly-X Technology

AdvanceBio Gly-X N-glycan prep and with 2-AB Express kit, 24-ct (formerly ProZyme) utilizes a novel in-solution enzymatic protein deglycosylation followed by on-matrix Express on-matrix labeling of released N-glycans with 2-AB dye, with no prior drydown. With deglycosylation carried out in solution, the method is simple, rapid, and suitable for automation. After a simple cleanup step, the 2-AB-labeled glycan samples are ready for analysis by UHPLC, LC/MS, MS/MS and other methods. Includes GX24-100 Gly-X deglycosylation module, GX96-401 Gly-X 2-AB Express labeling module, GX96-402 Gly-X 2-AB Express cleanup module (96-ct).

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Specifications
Application
  • Reductive amination 2-AB labeling chemistry without dry down
  • 5-minute N-Glycanase (PNGase F) digestion provides unbiased N-glycan release
  • Flexible, high-throughput format: process 1 to 24 samples
  • Optimized cleanup removes excess free dye, protein, and other interfering compounds.
Kit Configuration
  • Sample Prep Kit
Count
  • 24
Recovery
  • 80 %
Protein Load Range
  • 1-40 µg
Technique
  • LC & LC/MS
Deglycosylation Efficiency
  • 99 %
Prep time (approx)
  • ~2.5 hours
Stability
  • At least 1 month at -20°C, 5 days at 4–8°C
Equipment and Reagents Provided by User
  • 96-well Thermocycler or two independent heat blocks
  • Thermocycler (Corning THERM-1001, 110V
  • THERM-1000, 230V)
  • Vacuum manifold (Millipore MSVMHTS00-HTS)
  • Vacuum pump (Millipore WP6211560, 110 V
  • WP6122050, 220V
  • Welch WOB-L Pump 2522)
  • Vacuum manifold spacer (GX100, or GX400 Gly-X 2-AB Starter Kit - included free of charge with first Gly-X order, please contact us for details)
  • Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended)
  • DI water
Preservation of Sialylated Species
  • 99%
Resources

AdvanceBio Gly-X Deglycosylation and 2-AB Labeling Module set, 24-ct

Gly-X Deglycosylation Plate, Gly-X-Glycanase, 1 mg/mL, 30 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, 2-AB Dye Solution, 50 μl, 2-AB Reductant, 50 μl, 2-AB Catalyst, 100 μl, 2-AB Finishing Reagent, 230 μl

AdvanceBio Gly-X Deglycosylation and 2-AB Labeling Module set, 24-ct

Gly-X Deglycosylation Plate, Gly-X-Glycanase, 1 mg/mL, 30 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, 2-AB Dye Solution, 50 μl, 2-AB Reductant, 50 μl, 2-AB Catalyst, 100 μl, 2-AB Finishing Reagent, 230 μl

AdvanceBio Gly-X Deglycosylation and 2-AB Labeling Module set, 24-ct

Gly-X Deglycosylation Plate, Gly-X-Glycanase, 1 mg/mL, 30 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, 2-AB Dye Solution, 50 μl, 2-AB Reductant, 50 μl, 2-AB Catalyst, 100 μl, 2-AB Finishing Reagent, 230 μl

AdvanceBio Gly-X Deglycosylation and 2-AB Labeling Module set, 24-ct

Gly-X Deglycosylation Plate, Gly-X-Glycanase, 1 mg/mL, 30 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, 2-AB Dye Solution, 50 μl, 2-AB Reductant, 50 μl, 2-AB Catalyst, 100 μl, 2-AB Finishing Reagent, 230 μl

AdvanceBio Gly-X Deglycosylation and 2-AB Labeling Module set, 24-ct

Gly-X Deglycosylation Plate, Gly-X-Glycanase, 1 mg/mL, 30 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, 2-AB Dye Solution, 50 μl, 2-AB Reductant, 50 μl, 2-AB Catalyst, 100 μl, 2-AB Finishing Reagent, 230 μl

AdvanceBio Gly-X Deglycosylation and 2-AB Labeling Module set, 24-ct

Gly-X Deglycosylation Plate, Gly-X-Glycanase, 1 mg/mL, 30 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, 2-AB Dye Solution, 50 μl, 2-AB Reductant, 50 μl, 2-AB Catalyst, 100 μl, 2-AB Finishing Reagent, 230 μl

AdvanceBio Gly-X Deglycosylation and 2-AB Labeling Module set, 24-ct

Gly-X Deglycosylation Plate, Gly-X-Glycanase, 1 mg/mL, 30 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, 2-AB Dye Solution, 50 μl, 2-AB Reductant, 50 μl, 2-AB Catalyst, 100 μl, 2-AB Finishing Reagent, 230 μl

AdvanceBio Gly-X Deglycosylation and 2-AB Labeling Module set, 24-ct

Gly-X Deglycosylation Plate, Gly-X-Glycanase, 1 mg/mL, 30 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, 2-AB Dye Solution, 50 μl, 2-AB Reductant, 50 μl, 2-AB Catalyst, 100 μl, 2-AB Finishing Reagent, 230 μl

AdvanceBio Gly-X Deglycosylation and 2-AB Labeling Module set, 24-ct

Gly-X Deglycosylation Plate, Gly-X-Glycanase, 1 mg/mL, 30 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, 2-AB Dye Solution, 50 μl, 2-AB Reductant, 50 μl, 2-AB Catalyst, 100 μl, 2-AB Finishing Reagent, 230 μl

AdvanceBio Gly-X Deglycosylation and 2-AB Labeling Module set, 24-ct

Gly-X Deglycosylation Plate, Gly-X-Glycanase, 1 mg/mL, 30 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, 2-AB Dye Solution, 50 μl, 2-AB Reductant, 50 μl, 2-AB Catalyst, 100 μl, 2-AB Finishing Reagent, 230 μl

AdvanceBio Gly-X Deglycosylation and 2-AB Labeling Module set, 24-ct

Gly-X Deglycosylation Plate, Gly-X-Glycanase, 1 mg/mL, 30 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, 2-AB Dye Solution, 50 μl, 2-AB Reductant, 50 μl, 2-AB Catalyst, 100 μl, 2-AB Finishing Reagent, 230 μl

AdvanceBio Gly-X Deglycosylation and 2-AB Labeling Module set, 24-ct

Gly-X Deglycosylation Plate, Gly-X-Glycanase, 1 mg/mL, 30 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, 2-AB Dye Solution, 50 μl, 2-AB Reductant, 50 μl, 2-AB Catalyst, 100 μl, 2-AB Finishing Reagent, 230 μl

AdvanceBio Gly-X Deglycosylation and 2-AB Labeling Module set, 24-ct

Gly-X Deglycosylation Plate, Gly-X-Glycanase, 1 mg/mL, 30 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, 2-AB Dye Solution, 50 μl, 2-AB Reductant, 50 μl, 2-AB Catalyst, 100 μl, 2-AB Finishing Reagent, 230 μl

AdvanceBio Gly-X Deglycosylation and 2-AB Labeling Module set, 24-ct

Gly-X Deglycosylation Plate, Gly-X-Glycanase, 1 mg/mL, 30 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, 2-AB Dye Solution, 50 μl, 2-AB Reductant, 50 μl, 2-AB Catalyst, 100 μl, 2-AB Finishing Reagent, 230 μl

AdvanceBio Gly-X Deglycosylation and 2-AB Labeling Module set, 24-ct

Gly-X Deglycosylation Plate, Gly-X-Glycanase, 1 mg/mL, 30 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, 2-AB Dye Solution, 50 μl, 2-AB Reductant, 50 μl, 2-AB Catalyst, 100 μl, 2-AB Finishing Reagent, 230 μl

AdvanceBio Gly-X Deglycosylation and 2-AB Labeling Module set, 24-ct

Gly-X Deglycosylation Plate, Gly-X-Glycanase, 1 mg/mL, 30 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, 2-AB Dye Solution, 50 μl, 2-AB Reductant, 50 μl, 2-AB Catalyst, 100 μl, 2-AB Finishing Reagent, 230 μl