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Part Number:
GX24-201PC

AdvanceBio Gly-X Technology

AdvanceBio Gly-X N-glycan preparation and labeling with InstantPC module set, 24-ct (formerly ProZyme) utilizes a novel in-solution enzymatic protein deglycosylation followed by rapid labeling of released N-glycans with InstantPC dye. With deglycosylation and labeling carried out in solution phase, the method is simple, rapid, and suitable for automation. The InstantPC dye delivers unmatched fluorescent brightness and MS signal, which enables a single labeling method to be deployed across different glycan analysis workflows. Includes GX24-100 Gly-X Deglycosylation Module, GX24-101 Gly-X InstantPC Labeling Module.

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Specifications
Application
  • Small molecule size for improved chromatographic peak resolution
  • Flexible, high-throughput format: process 1 to 24 samples
  • Reconstituted InstantPC dye may be stored frozen (-20°C) for up to 3 months.
Kit Configuration
  • Sample Prep Module Set
Count
  • 24
Recovery
  • 95 %
Protein Load Range
  • 1-40 µg
Technique
  • LC & LC/MS
Deglycosylation Efficiency
  • Complete for most proteins. Protein load range: *More than 40 μg glycoprotein may be used, depending on the extent of glycosylation. %
Prep time (approx)
  • 42 min (16 samples), 99 min (96 samples)
Stability
  • Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC Eluent or after dilution with ACN/DMF
Equipment and Reagents Provided by User
  • 96-well Thermocycler or two independent heat blocks, set for 90 °C and 50 °C
  • Thermocycler (Corning THERM-1001, 110V
  • THERM-1000, 230V)
  • Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks
Preservation of Sialylated Species
  • 95%
Resources

AdvanceBio Gly-X Deglycosylation and InstantPC Labeling Module set, 24-ct

Gly-X Deglycosylation Plate,Gly-X-Glycanase, 1 mg/mL, 30 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, InstantPC Dye, 30mg, InstantPC Dye Solvent, 0.6ml

AdvanceBio Gly-X Deglycosylation and InstantPC Labeling Module set, 24-ct

Gly-X Deglycosylation Plate,Gly-X-Glycanase, 1 mg/mL, 30 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, InstantPC Dye, 30mg, InstantPC Dye Solvent, 0.6ml

AdvanceBio Gly-X Deglycosylation and InstantPC Labeling Module set, 24-ct

Gly-X Deglycosylation Plate,Gly-X-Glycanase, 1 mg/mL, 30 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, InstantPC Dye, 30mg, InstantPC Dye Solvent, 0.6ml

AdvanceBio Gly-X Deglycosylation and InstantPC Labeling Module set, 24-ct

Gly-X Deglycosylation Plate,Gly-X-Glycanase, 1 mg/mL, 30 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, InstantPC Dye, 30mg, InstantPC Dye Solvent, 0.6ml

AdvanceBio Gly-X Deglycosylation and InstantPC Labeling Module set, 24-ct

Gly-X Deglycosylation Plate,Gly-X-Glycanase, 1 mg/mL, 30 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, InstantPC Dye, 30mg, InstantPC Dye Solvent, 0.6ml

AdvanceBio Gly-X Deglycosylation and InstantPC Labeling Module set, 24-ct

Gly-X Deglycosylation Plate,Gly-X-Glycanase, 1 mg/mL, 30 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, InstantPC Dye, 30mg, InstantPC Dye Solvent, 0.6ml

AdvanceBio Gly-X Deglycosylation and InstantPC Labeling Module set, 24-ct

Gly-X Deglycosylation Plate,Gly-X-Glycanase, 1 mg/mL, 30 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, InstantPC Dye, 30mg, InstantPC Dye Solvent, 0.6ml

AdvanceBio Gly-X Deglycosylation and InstantPC Labeling Module set, 24-ct

Gly-X Deglycosylation Plate,Gly-X-Glycanase, 1 mg/mL, 30 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, InstantPC Dye, 30mg, InstantPC Dye Solvent, 0.6ml

AdvanceBio Gly-X Deglycosylation and InstantPC Labeling Module set, 24-ct

Gly-X Deglycosylation Plate,Gly-X-Glycanase, 1 mg/mL, 30 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, InstantPC Dye, 30mg, InstantPC Dye Solvent, 0.6ml

AdvanceBio Gly-X Deglycosylation and InstantPC Labeling Module set, 24-ct

Gly-X Deglycosylation Plate,Gly-X-Glycanase, 1 mg/mL, 30 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, InstantPC Dye, 30mg, InstantPC Dye Solvent, 0.6ml

AdvanceBio Gly-X Deglycosylation and InstantPC Labeling Module set, 24-ct

Gly-X Deglycosylation Plate,Gly-X-Glycanase, 1 mg/mL, 30 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, InstantPC Dye, 30mg, InstantPC Dye Solvent, 0.6ml

AdvanceBio Gly-X Deglycosylation and InstantPC Labeling Module set, 24-ct

Gly-X Deglycosylation Plate,Gly-X-Glycanase, 1 mg/mL, 30 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, InstantPC Dye, 30mg, InstantPC Dye Solvent, 0.6ml

AdvanceBio Gly-X Deglycosylation and InstantPC Labeling Module set, 24-ct

Gly-X Deglycosylation Plate,Gly-X-Glycanase, 1 mg/mL, 30 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, InstantPC Dye, 30mg, InstantPC Dye Solvent, 0.6ml

AdvanceBio Gly-X Deglycosylation and InstantPC Labeling Module set, 24-ct

Gly-X Deglycosylation Plate,Gly-X-Glycanase, 1 mg/mL, 30 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, InstantPC Dye, 30mg, InstantPC Dye Solvent, 0.6ml

AdvanceBio Gly-X Deglycosylation and InstantPC Labeling Module set, 24-ct

Gly-X Deglycosylation Plate,Gly-X-Glycanase, 1 mg/mL, 30 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, InstantPC Dye, 30mg, InstantPC Dye Solvent, 0.6ml

AdvanceBio Gly-X Deglycosylation and InstantPC Labeling Module set, 24-ct

Gly-X Deglycosylation Plate,Gly-X-Glycanase, 1 mg/mL, 30 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, InstantPC Dye, 30mg, InstantPC Dye Solvent, 0.6ml