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Part Number:
GS48-SAQ

AdvanceBio Sialic Acid Quantitation

AdvanceBio total Sialic acid quantitation kit, 48-ct (formerly ProZyme). Rapid quantitation of total sialic acid by fluorescence or absorbance detection, either free or released from intact glycoproteins. Automatable, 96-well microplate format with fluorescence detection. Broad range of detection, 40 pmol - 1,000 pmol. Sample digestion, conversion, detection and quantitation may be performed in a single well for fast and simple processing. Enzymatic cleavage allows rapid analysis (~75 minutes or less) with minimal, degradation of sialic acid.

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$800.00

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Specifications
Application
  • Automatable, 96-well microplate format with fluorescence detection
  • Broad range of detection, 40 pmol - 1,000 pmol
  • Sample digestion, conversion, detection and quantitation may be performed in a single well for fast and simple processing
  • Enzymatic cleavage allows rapid analysis (~75 minutes or less) with minimal, if any, degradation of sialic acid
Count
  • 48
Prep time (approx)
  • ~75 minutes
Equipment and Reagents Provided by User
  • Laboratory oven or block heater capable of 37°C incubation.
  • Heat block with a flat surface to accept a 96-well skirted plate (e.g. VWR 13259-295 modular heating block for titer plate).
  • Plate reader for measurement by fluorescence detection (530 nm excitation, 590 nm emission), or absorbance (530 nm).
  • Optional plate reader with 37°C temperature control (no need for the heater and block).
Resources

AdvanceBio Total Sialic Acid Quantitation Kit, 48-ct

AdvanceBio Sialidase A, 1 U, 0.4 ml, SAQ Dye, 0.064 mg, Horseradish Peroxidase, 60 μl, Conversion Reagents, 120 μl, SAQ Buffer A, 3 ml, SAQ Buffer B, 1 ml, SAQ Buffer C, 0.3 ml, Sialic Acid Standard, 1 ml, DMSO, 0.35 ml, Bovine Fetuin Control, 0.4 mg

AdvanceBio Total Sialic Acid Quantitation Kit, 48-ct

AdvanceBio Sialidase A, 1 U, 0.4 ml, SAQ Dye, 0.064 mg, Horseradish Peroxidase, 60 μl, Conversion Reagents, 120 μl, SAQ Buffer A, 3 ml, SAQ Buffer B, 1 ml, SAQ Buffer C, 0.3 ml, Sialic Acid Standard, 1 ml, DMSO, 0.35 ml, Bovine Fetuin Control, 0.4 mg

AdvanceBio Total Sialic Acid Quantitation Kit, 48-ct

AdvanceBio Sialidase A, 1 U, 0.4 ml, SAQ Dye, 0.064 mg, Horseradish Peroxidase, 60 μl, Conversion Reagents, 120 μl, SAQ Buffer A, 3 ml, SAQ Buffer B, 1 ml, SAQ Buffer C, 0.3 ml, Sialic Acid Standard, 1 ml, DMSO, 0.35 ml, Bovine Fetuin Control, 0.4 mg

AdvanceBio Total Sialic Acid Quantitation Kit, 48-ct

AdvanceBio Sialidase A, 1 U, 0.4 ml, SAQ Dye, 0.064 mg, Horseradish Peroxidase, 60 μl, Conversion Reagents, 120 μl, SAQ Buffer A, 3 ml, SAQ Buffer B, 1 ml, SAQ Buffer C, 0.3 ml, Sialic Acid Standard, 1 ml, DMSO, 0.35 ml, Bovine Fetuin Control, 0.4 mg

AdvanceBio Total Sialic Acid Quantitation Kit, 48-ct

AdvanceBio Sialidase A, 1 U, 0.4 ml, SAQ Dye, 0.064 mg, Horseradish Peroxidase, 60 μl, Conversion Reagents, 120 μl, SAQ Buffer A, 3 ml, SAQ Buffer B, 1 ml, SAQ Buffer C, 0.3 ml, Sialic Acid Standard, 1 ml, DMSO, 0.35 ml, Bovine Fetuin Control, 0.4 mg

AdvanceBio Total Sialic Acid Quantitation Kit, 48-ct

AdvanceBio Sialidase A, 1 U, 0.4 ml, SAQ Dye, 0.064 mg, Horseradish Peroxidase, 60 μl, Conversion Reagents, 120 μl, SAQ Buffer A, 3 ml, SAQ Buffer B, 1 ml, SAQ Buffer C, 0.3 ml, Sialic Acid Standard, 1 ml, DMSO, 0.35 ml, Bovine Fetuin Control, 0.4 mg

AdvanceBio Total Sialic Acid Quantitation Kit, 48-ct

AdvanceBio Sialidase A, 1 U, 0.4 ml, SAQ Dye, 0.064 mg, Horseradish Peroxidase, 60 μl, Conversion Reagents, 120 μl, SAQ Buffer A, 3 ml, SAQ Buffer B, 1 ml, SAQ Buffer C, 0.3 ml, Sialic Acid Standard, 1 ml, DMSO, 0.35 ml, Bovine Fetuin Control, 0.4 mg

AdvanceBio Total Sialic Acid Quantitation Kit, 48-ct

AdvanceBio Sialidase A, 1 U, 0.4 ml, SAQ Dye, 0.064 mg, Horseradish Peroxidase, 60 μl, Conversion Reagents, 120 μl, SAQ Buffer A, 3 ml, SAQ Buffer B, 1 ml, SAQ Buffer C, 0.3 ml, Sialic Acid Standard, 1 ml, DMSO, 0.35 ml, Bovine Fetuin Control, 0.4 mg

AdvanceBio Total Sialic Acid Quantitation Kit, 48-ct

AdvanceBio Sialidase A, 1 U, 0.4 ml, SAQ Dye, 0.064 mg, Horseradish Peroxidase, 60 μl, Conversion Reagents, 120 μl, SAQ Buffer A, 3 ml, SAQ Buffer B, 1 ml, SAQ Buffer C, 0.3 ml, Sialic Acid Standard, 1 ml, DMSO, 0.35 ml, Bovine Fetuin Control, 0.4 mg

AdvanceBio Total Sialic Acid Quantitation Kit, 48-ct

AdvanceBio Sialidase A, 1 U, 0.4 ml, SAQ Dye, 0.064 mg, Horseradish Peroxidase, 60 μl, Conversion Reagents, 120 μl, SAQ Buffer A, 3 ml, SAQ Buffer B, 1 ml, SAQ Buffer C, 0.3 ml, Sialic Acid Standard, 1 ml, DMSO, 0.35 ml, Bovine Fetuin Control, 0.4 mg

AdvanceBio Total Sialic Acid Quantitation Kit, 48-ct

AdvanceBio Sialidase A, 1 U, 0.4 ml, SAQ Dye, 0.064 mg, Horseradish Peroxidase, 60 μl, Conversion Reagents, 120 μl, SAQ Buffer A, 3 ml, SAQ Buffer B, 1 ml, SAQ Buffer C, 0.3 ml, Sialic Acid Standard, 1 ml, DMSO, 0.35 ml, Bovine Fetuin Control, 0.4 mg

AdvanceBio Total Sialic Acid Quantitation Kit, 48-ct

AdvanceBio Sialidase A, 1 U, 0.4 ml, SAQ Dye, 0.064 mg, Horseradish Peroxidase, 60 μl, Conversion Reagents, 120 μl, SAQ Buffer A, 3 ml, SAQ Buffer B, 1 ml, SAQ Buffer C, 0.3 ml, Sialic Acid Standard, 1 ml, DMSO, 0.35 ml, Bovine Fetuin Control, 0.4 mg

AdvanceBio Total Sialic Acid Quantitation Kit, 48-ct

AdvanceBio Sialidase A, 1 U, 0.4 ml, SAQ Dye, 0.064 mg, Horseradish Peroxidase, 60 μl, Conversion Reagents, 120 μl, SAQ Buffer A, 3 ml, SAQ Buffer B, 1 ml, SAQ Buffer C, 0.3 ml, Sialic Acid Standard, 1 ml, DMSO, 0.35 ml, Bovine Fetuin Control, 0.4 mg

AdvanceBio Total Sialic Acid Quantitation Kit, 48-ct

AdvanceBio Sialidase A, 1 U, 0.4 ml, SAQ Dye, 0.064 mg, Horseradish Peroxidase, 60 μl, Conversion Reagents, 120 μl, SAQ Buffer A, 3 ml, SAQ Buffer B, 1 ml, SAQ Buffer C, 0.3 ml, Sialic Acid Standard, 1 ml, DMSO, 0.35 ml, Bovine Fetuin Control, 0.4 mg