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GlykoPrep-plus Technology

GlykoPrep-plus N-glycan sample preparation with 2-AB, 96-ct complete kit (formerly ProZyme). Configured for automation on the Agilent AssayMAP Bravo liquid handling platform. Includes GSP-RX digestion module, GS96-AB Reductive-Amination 2-AB labeling module, GSP-CU cleanup module, AM96-NG GlykoPrep-plus labware set.

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Instrument Compatibility
  • AssayMAP Bravo Platform
Kit Configuration
  • Sample Prep Kit
  • 96
  • LC & LC/MS
Prep time (approx)
  • 3 hours
Equipment and Reagents Provided by User
  • Ultrapure, deionized water (~100 mL, Milli-Q or equivalent)
  • 100% Acetonitrile (~250 mL, HPLC-grade)
  • Microplate compatible, centrifugal evaporator (e.g., SpeedVac with plate rotor or similar)
  • Fume hood
  • Vortexer
  • Nitrile gloves
  • Calibrated pipettes and disposable tips (P5/10, P200 and P1000)
  • Volumetric pipettes (5 mL, 1 ea)
  • Glass graduated cylinder (50 mL, 1 ea)

GPPNG-AB Instruction Manual

User manual for GPPNG-AB Instruction Manual