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GlykoPrep Technology

GlykoPrep N-glycan sample preparation with InstantAB, 96-ct complete kit (formerly ProZyme) is a cartridge based workflow with rapid digestion, instant labeling, and cleanup of excess InstantAB label. Includes GS24-RX digestion module, GS24-LB InstantAB labeling module, GS24-CU cleanup module.

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  • Nonselective, rapid release and recovery of intact N-glycans
  • Flexible format
  • RT labeling with InstantAB with reduced loss of sialic acid compared to reductive amination labeling chemistry
  • labeling efficiency 80%
  • Purified, fluorescently labeled N-glycans are produced in water and ready for analysis without concentrating or drying
Kit Configuration
  • Sample Prep Kit
  • 96
  • LC
Prep time (approx)
  • 3 hours
Equipment and Reagents Provided by User
  • Heater and incubation blocks, capable of 50–100°C, available from Agilent (GS150)
  • AssayMAP Labware (AM200)
  • Centrifuge (capable of 50 - 1000 x g) and deep microplate rotor with clearance of minimum 44 mm height
  • Ultrapure, deionized water
  • Acetonitrile (ACN, 100%, HPLC-grade)
  • Formic acid (HPLC-grade)

InstantAB Microfuge Method

User manual for InstantAB Microfuge Method

GP96NG-LB GlykoPrep Rapid N-Glycan Preparation with InstantAB

User manual for TDW-GP96NG-LB GlykoPrep Rapid N-Glycan Preparation with InstantAB