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GlykoPrep Technology

GlykoPrep N-glycan sample preparation with 2-AB, 96-ct complete kit (formerly ProZyme) is a cartridge based workflow with rapid digestion, labeling, and cleanup of excess 2-AB label. Includes GS96-RX digestion module, GS96-AB Reductive-Amination 2-AB labeling module; GS96-CU cleanup module

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  • Nonselective, rapid release and recovery of intact N-glycans
  • Flexible, high-throughput format: process 1 to 192 samples per run (2 kits simultaneously)
  • Rapid-reductive-amination labeling with 2-AB
  • Purified, fluorescently labeled N-glycans are produced in water and ready for analysis without concentrating or drying
  • Compatible with microplate liquid handling on a broad range of automation platforms
Kit Configuration
  • Sample Prep Kit
  • 96
  • LC & LC/MS
Prep time (approx)
  • 4 hours
Equipment and Reagents Provided by User
  • Heater and incubation blocks, capable of 50–100°C, available from Agilent (GS150)
  • AssayMAP Labware (AM200)
  • Centrifuge (capable of 50 - 1000 x g) and deep microplate rotor with clearance of minimum 44 mm height
  • Ultrapure, deionized water
  • Acetonitrile (ACN, 100%, HPLC-grade)
  • Formic acid (HPLC-grade)

GlykoPrep Rapid N-Glycan Preparation with 2-AB Booklet

User manual for GP96NG-AB GlykoPrep Rapid N-Glycan Preparation with 2-AB