GKE-5020B
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Part Number:
GKE-5020B

Endoglycosidases

AdvanceBio N-Glycanase-ultra (PNGase F, EDTA free), 400 mU, ≥10 U/mL (formerly ProZyme). Releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. The same formulation provided in GlykoPrep, ≥ 10 U/mL and EDTA-free. Includes 5x N-Glycanase reaction buffer, denaturation solution, detergent solution

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List Price:
$1,087.00

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Specifications
Enzyme Specific Activity
  • ≥10 units/mg
Volume
  • 400 µL
pH Range
  • 7.5-9.5
Enzyme Specificity
  • Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless a(1-3) core fucosylated, as in plant glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate, and sialic acid groups attached to the oligosaccharide do not affect cleavage. Endo F free. Highly concentrated, particularly useful for deglycosylation under native conditions.
Enzyme Applications
  • To obtain efficient deglycosylation of glycoprotein substrates under non-denaturing conditions, it is necessary to use a higher starting concentration of enzyme. N-Glycanase® PLUS and ULTRA (EDTA-Free) are supplied at = 10 U/ml, and are recommended for all applications requiring deglycosylation of glycoproteins in the absence of denaturants. The high activity also allows smaller reaction volumes and shorter reaction times to be explored.
Enzyme Unit Definition
  • One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 µmole of denatured ribonuclease B per minute at 37°C, pH 7.5.
Enzyme Formulation
  • 20 mM Tris-HCl, 50 mM NaCl (pH 7.5, EDTA-free formulation).
Enzyme Source
  • Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as Endoglycosidase F2, endo-beta-N-acetylglucosaminidase. Enzyme also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase.
Concentration
  • 10 U/mL
Unit
  • 400 mU
Molecular Weight
  • ~35,000 Daltons
pH Optimum
  • 8.6
Resources

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AdvanceBio N-聚糖酶-ULTRA(PNGase F,不含 EDTA), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL