Please login to add product to your favorites.

Part Number:
GKE-5016B

Endoglycosidases

AdvanceBio N-Glycanase (PNGase F, EDTA free), 200 mU, ≥2.5 U/mL, (formerly ProZyme). Releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. Includes 5x N-Glycanase reaction buffer, denaturation solution, detergent solution, 5x N-Glycanase Tris reaction buffer (MS applications).

Item successfully added to your list
List Price:
$457.00

Please enter valid quantity

Specifications
Enzyme Specific Activity
  • ≥10 units/mg
Volume
  • 80 µL
pH Range
  • 7.5-9.5
Enzyme Specificity
  • Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless a(1-3) core fucosylated, as in plant glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate, and sialic acid groups attached to the oligosaccharide do not affect cleavage. Endo F free. Highly concentrated, particularly useful for deglycosylation under native conditions.
Enzyme Unit Definition
  • One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 µmole of denatured ribonuclease B per minute at 37°C, pH 7.5.
Enzyme Formulation
  • 20 mM Tris-HCl, 50 mM NaCl (pH 7.5)
Enzyme Source
  • Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as Endoglycosidase F2, endo-beta-N-acetylglucosaminidase. Enzyme also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase.
Concentration
  • 2.5 U/mL
Unit
  • 200 mU
Molecular Weight
  • ~35,000 Daltons
pH Optimum
  • 8.6
Resources

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-聚糖酶(PNGase F,不含 EDTA),≥ 2.5 U/ml

AdvanceBio N-聚糖酶(PNGase F,不含 EDTA),≥ 2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml