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Part Number:
GK80110

Endoglycosidases

The Enzymatic deglycosylation kit for N-linked and simple O-linked Glycans (formerly ProZyme) contains all enzymes and reagents needed to remove all N-linked glycans and simple O-linked glycans (including polysialylated) from glycoproteins. See the product manual for an example using bovine fetuin. N-Glycanase (PNGase F) cleaves all N-linked complex, hybrid or high mannose oligosaccharides unless α(1,3) core fucosylated (asparagine must be peptide-bonded at both termini). There is no such universal endoglycosidase for O-glycans. These have to be trimmed down to Gal-GalNAc-O-S/T before O-glycanase (GK80090) can remove the Gal-GalNAc structure. Modification of this core with, e.g., sialic acid will block O-glycanase. Sialidase A (GK80040) is included in the kit and can remove terminal sialic acid from sialylated T antigen/Core 1 (Sia-Gal-GalNAc-O-S/T) to reveal core 1 Gal-GalNAc. The GK80115 extender kit (β(1,4)Galactosidase and β-N-Acetylglucosaminidase), available separately, can assist with removal of e.g. core 2 structures (GlcNAc-Gal-GalNAc-S/T). For more extended and complex O-glycans structures these enzymes will not be sufficient to reveal Gal-GalNAc. For more on O-GalNAc glycan core structures, please see references

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List Price:
$359.00

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Specifications
Application
  • Epitope and binding site analysis
Enzyme Applications
  • Deglycosylates up to 2 mg of glycoprotein
  • Native and denaturing protocols
  • No degradation of protein
  • Removes O-linked sugars containing polysialic acid
  • C Control glycoprotein provided
Resources

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked & Simple O-Linked Glycans, 113 002, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked & Simple O-Linked Glycans, 113 002, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 091, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 091, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 090, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 090, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 086, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 086, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 087, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 087, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 082, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 082, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 093, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 093, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 094, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 094, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 080, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 080, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 083, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 083, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 084, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 084, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 079, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 079, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 092, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 092, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 088, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 088, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 089, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 089, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked & Simple O-Linked Glycans, 113 001, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked & Simple O-Linked Glycans, 113 001, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 081, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 081, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 085, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 085, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked & Simple O-Linked Glycans, DG26 095, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked & Simple O-Linked Glycans, DG26 095, Certificate of Analysis

Enzymatic Deglycosylation Kit for N-Linked & Simple O-Linked Glycans

Fetuin Control Protein, 0.500 mg, AdvanceBio N-Glycanase, 20 μl, AdvanceBio Sialidase A, 20 μl, AdvanceBio O-Glycanase, 20 μl, 5x Reaction Buffer for GK80110, 1 ml, Denaturation Solution, 500 μl, Detergent solution (15% Detergent Solution), 200 μl

Enzymatic Deglycosylation Kit for N-Linked & Simple O-Linked Glycans

Fetuin Control Protein, 0.500 mg, AdvanceBio N-Glycanase, 20 μl, AdvanceBio Sialidase A, 20 μl, AdvanceBio O-Glycanase, 20 μl, 5x Reaction Buffer for GK80110, 1 ml, Denaturation Solution, 500 μl, Detergent solution (15% Detergent Solution), 200 μl

Enzymatic Deglycosylation Kit for N-Linked & Simple O-Linked Glycans

Fetuin Control Protein, 0.500 mg, AdvanceBio N-Glycanase, 20 μl, AdvanceBio Sialidase A, 20 μl, AdvanceBio O-Glycanase, 20 μl, 5x Reaction Buffer for GK80110, 1 ml, Denaturation Solution, 500 μl, Detergent solution (15% Detergent Solution), 200 μl

Enzymatic Deglycosylation Kit for N-Linked & Simple O-Linked Glycans

Fetuin Control Protein, 0.500 mg, AdvanceBio N-Glycanase, 20 μl, AdvanceBio Sialidase A, 20 μl, AdvanceBio O-Glycanase, 20 μl, 5x Reaction Buffer for GK80110, 1 ml, Denaturation Solution, 500 μl, Detergent solution (15% Detergent Solution), 200 μl

Enzymatic Deglycosylation Kit for N-Linked & Simple O-Linked Glycans

Fetuin Control Protein, 0.500 mg, AdvanceBio N-Glycanase, 20 μl, AdvanceBio Sialidase A, 20 μl, AdvanceBio O-Glycanase, 20 μl, 5x Reaction Buffer for GK80110, 1 ml, Denaturation Solution, 500 μl, Detergent solution (15% Detergent Solution), 200 μl

Enzymatic Deglycosylation Kit for N-Linked & Simple O-Linked Glycans

Fetuin Control Protein, 0.500 mg, AdvanceBio N-Glycanase, 20 μl, AdvanceBio Sialidase A, 20 μl, AdvanceBio O-Glycanase, 20 μl, 5x Reaction Buffer for GK80110, 1 ml, Denaturation Solution, 500 μl, Detergent solution (15% Detergent Solution), 200 μl

Enzymatic Deglycosylation Kit for N-Linked & Simple O-Linked Glycans

Fetuin Control Protein, 0.500 mg, AdvanceBio N-Glycanase, 20 μl, AdvanceBio Sialidase A, 20 μl, AdvanceBio O-Glycanase, 20 μl, 5x Reaction Buffer for GK80110, 1 ml, Denaturation Solution, 500 μl, Detergent solution (15% Detergent Solution), 200 μl

Enzymatic Deglycosylation Kit for N-Linked & Simple O-Linked Glycans

Fetuin Control Protein, 0.500 mg, AdvanceBio N-Glycanase, 20 μl, AdvanceBio Sialidase A, 20 μl, AdvanceBio O-Glycanase, 20 μl, 5x Reaction Buffer for GK80110, 1 ml, Denaturation Solution, 500 μl, Detergent solution (15% Detergent Solution), 200 μl

Enzymatic Deglycosylation Kit for N-Linked & Simple O-Linked Glycans

Fetuin Control Protein, 0.500 mg, AdvanceBio N-Glycanase, 20 μl, AdvanceBio Sialidase A, 20 μl, AdvanceBio O-Glycanase, 20 μl, 5x Reaction Buffer for GK80110, 1 ml, Denaturation Solution, 500 μl, Detergent solution (15% Detergent Solution), 200 μl

Enzymatic Deglycosylation Kit for N-Linked & Simple O-Linked Glycans

Fetuin Control Protein, 0.500 mg, AdvanceBio N-Glycanase, 20 μl, AdvanceBio Sialidase A, 20 μl, AdvanceBio O-Glycanase, 20 μl, 5x Reaction Buffer for GK80110, 1 ml, Denaturation Solution, 500 μl, Detergent solution (15% Detergent Solution), 200 μl

Enzymatic Deglycosylation Kit for N-Linked & Simple O-Linked Glycans

Fetuin Control Protein, 0.500 mg, AdvanceBio N-Glycanase, 20 μl, AdvanceBio Sialidase A, 20 μl, AdvanceBio O-Glycanase, 20 μl, 5x Reaction Buffer for GK80110, 1 ml, Denaturation Solution, 500 μl, Detergent solution (15% Detergent Solution), 200 μl

Enzymatic Deglycosylation Kit for N-Linked & Simple O-Linked Glycans

Fetuin Control Protein, 0.500 mg, AdvanceBio N-Glycanase, 20 μl, AdvanceBio Sialidase A, 20 μl, AdvanceBio O-Glycanase, 20 μl, 5x Reaction Buffer for GK80110, 1 ml, Denaturation Solution, 500 μl, Detergent solution (15% Detergent Solution), 200 μl

Enzymatic Deglycosylation Kit for N-Linked & Simple O-Linked Glycans

Fetuin Control Protein, 0.500 mg, AdvanceBio N-Glycanase, 20 μl, AdvanceBio Sialidase A, 20 μl, AdvanceBio O-Glycanase, 20 μl, 5x Reaction Buffer for GK80110, 1 ml, Denaturation Solution, 500 μl, Detergent solution (15% Detergent Solution), 200 μl

Enzymatic Deglycosylation Kit for N-Linked & Simple O-Linked Glycans

Fetuin Control Protein, 0.500 mg, AdvanceBio N-Glycanase, 20 μl, AdvanceBio Sialidase A, 20 μl, AdvanceBio O-Glycanase, 20 μl, 5x Reaction Buffer for GK80110, 1 ml, Denaturation Solution, 500 μl, Detergent solution (15% Detergent Solution), 200 μl

Enzymatic Deglycosylation Kit for N-Linked & Simple O-Linked Glycans

Fetuin Control Protein, 0.500 mg, AdvanceBio N-Glycanase, 20 μl, AdvanceBio Sialidase A, 20 μl, AdvanceBio O-Glycanase, 20 μl, 5x Reaction Buffer for GK80110, 1 ml, Denaturation Solution, 500 μl, Detergent solution (15% Detergent Solution), 200 μl

Enzymatic Deglycosylation Kit for N-Linked & Simple O-Linked Glycans

Fetuin Control Protein, 0.500 mg, AdvanceBio N-Glycanase, 20 μl, AdvanceBio Sialidase A, 20 μl, AdvanceBio O-Glycanase, 20 μl, 5x Reaction Buffer for GK80110, 1 ml, Denaturation Solution, 500 μl, Detergent solution (15% Detergent Solution), 200 μl