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SureMASTR BRCA Screen

Part Number:
MF-0360.FQ
RUO

Product is currently out of stock. MF_SureMASTR BRCA Screen_FQ. Service: FASTQ generation SureMASTR BRCA Screen. From DNA sample to FASTQ file.

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Specifications
Amplicon Length
  • 80-224 bp
Sample Input Size
  • Germline: 50 ng per multiplex reaction
  • Somatic: 8 - 250 ng per multiplex reaction
Number of Plexes
  • 1
Number of Reactions
  • 24
Number of Samples per Run
  • Germline: 50 ng per multiplex reaction
  • Somatic: 8 - 250 ng per multiplex reaction
  • 50 % VAFsample (20 reads/allele):
  • - MiSeq Reagent Nano Kit v2 (300 and 500 cycles): 10
  • - MiSeq Reagent Micro Kit v2 (300 cycles): 42
  • - MiSeq Reagent Kit v2 (300 and 500 cycles): 160
  • - MiSeq Reagent Kit v3 (600 cycles): 294*
  • - MiniSeq Mid Output Kit (300 cycles): 93
  • - MiniSeq High Output Kit (300 cycles): 294*
  • - NextSeq 500/550 Mid Output Kit v2 (300 cycles): 1319*
  • - NextSeq 500/550 High Output Kit v2 (300 cycles): 4281*
  • 5 % VAFsample (20 reads/allele):
  • - MiSeq Reagent Kit v2 (300 and 500 cycles): 16
  • - MiSeq Reagent Kit v3 (600 cycles): 29
  • - MiniSeq Mid Output Kit (300 cycles): 9
  • - MiniSeq High Output Kit (300 cycles): 29
  • - NextSeq 500/550 Mid Output Kit v2 (300 cycles): 139
  • - NextSeq 500/550 High Output Kit v2 (300 cycles): 428*
Number of Amplicons
  • 355
Compatible Sequencers
  • Illumina MiSeq
  • Illumina MiniSeq
  • Illumina NextSeq
  • Illumina MiSeq v2
  • Illumina MiSeq v3
Targeted Genes
  • BRCA1
  • BRCA2
  • CHEK2
  • PALB2
Target Region
  • 28.4 Kb
Tissue Sample Type
  • FFPE
  • Blood
  • FFT