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GlykoPrep Technology

Part Number:
GP96NG-PC

GlykoPrep N-glycan prep with InstantPC, 96-ct (formerly ProZyme). Complete kit, GlykoPrep N-glycan sample preparation is a cartridge based workflow with rapid digestion, instant labeling, and cleanup of excess InstantPC label. The InstantPC dye delivers unmatched fluorescent brightness and MS signal, which enables a single labeling method to be deployed across different glycan analysis workflows. Includes GS96-RX digestion module, GS96-PC InstantPC labeling module, GS96-CU cleanup module.

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List Price:
$7,005.00 /96 Pack

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Specifications
Equipment and Reagents Provided by User
  • Heater and incubation blocks, capable of 50–100°C, available from Agilent (GS150)
  • AssayMAP Labware (AM200)
  • Centrifuge (capable of 50 - 1000 x g) and deep microplate rotor with clearance of minimum 44 mm height
  • Ultrapure, deionized water
  • Acetonitrile (ACN, 100%, HPLC-grade)
  • Formic acid (HPLC-grade)
Label
  • InstantPC
Prep time (approx)
  • 3 hours
Application
    Heater and incubation blocks, capable of 50–100°C, available from Agilent (GS150)
  • AssayMAP Labware (AM200)
  • Centrifuge (capable of 50 - 1000 x g) and deep microplate rotor with clearance of minimum 44 mm height
  • Ultrapure, deionized water
  • Acetonitrile (ACN, 100%, HPLC-grade)
  • Formic acid (HPLC-grade)
  • Flexible, high-throughput format: process 1 to 192 samples per run (2 kits simultaneously)
Count
  • 96
Technique
  • LC & LC/MS
Kit Configuration
  • Sample Prep Kit
Kit Contents
Part Number Product Description Quantity
GS96-RX GlykoPrep digestion module, 96-ct (formerly ProZyme) 1
GS96-PC GlykoPrep InstantPC labeling module, 96-ct (formerly ProZyme) 1
GS96-CU GlykoPrep cleanup module, 96-ct (formerly ProZyme) 1
Resources

Comparison of Relative Quantification of Monoclonal Antibody N-glycans Using Fluorescence and MS Detection

Comparison of Relative Quantification of Monoclonal Antibody N-glycans Using Fluorescence and MS Detection

InstantPC Microfuge Method

User manual for Microfuge IPC

Profiling Glycosylation of Monoclonal Antibodies at Three Levels Using the Agilent 6545XT AdvanceBio LC/Q-TOF

This application note demonstrates three approaches to quantitatively understand the glycosylation present for a given mAb.

2-AB Microfuge Method

User manual for 2-AB Microfuge Method