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DNase I, RNase Free

Part Number:
600032

CRNase Free DNase I; contains mixture of four isoenzymes from bovine pancreas (10 units/µL)

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List Price:
$269.00

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Specifications
Reaction Conditions
  • 40 mM Tris-HCl (pH 7.5), 6 mM MgCl2, 2 mM CaCl2, 1 µg DNA and 1 unit enzyme in a 25 µL volume. Incubate 30 minutes at 37°C.
Reactions per Kit
  • 5000 units
Storage Buffer
  • 10mM Tris-HCl (pH 7.5), 10 mM CaCl2, 10 mM MgCl2 and 50 % glycerol (v/v) (Dilutions should be made in this buffer.)
Incubation Time
  • Incubate 30 minutes at 37 °C
Physical Form
  • Premixed
Substrate Type
  • RNA
Concentration
  • 10 U/µL
Unit Definition
  • One unit will completely digest 1 µg of DNA in 10 minutes at 37°C in a 25 µL reaction volume.