PRESENTATION

In alignment with FDA’s priority focused on new approach methodologies (NAMs), Agilent developed an assay workflow to interrogate energy metabolism and assess mitochondrial function in organoid cultures using the Seahorse XF Flex organoid microplate and the Seahorse XF Flex analyzer. This workflow empowers physiologically relevant discoveries in a wide range of biomedical research areas.

• How to establish organoid cultures in the XF Flex organoid microplate and perform Seahorse XF assays.
• Techniques for optimizing assay performance with different types of organoid cultures and data normalization options
• Examples demonstrating the applicability of the XF Flex organoid workflow in cancer research and drug discovery as well as in drug safety studies.
• A summary of additional XF Flex workflows compatible with other 3D models