PRESENTATION

Wednesday, January 24, 2024

Confocal light microscopy is a powerful tool that, by reducing out-of-focus light, provides three-dimensional insight into biological processes.

Combined with high numerical aperture water immersion objectives, image quality is further improved by more efficient light collection and correcting for light distortion associated with refractive index mismatches.

In this webinar, we introduce two new features of the Agilent BioTek Cytation C10 confocal imaging reader: water immersion imaging, which allows for more efficient, gentle detection of dim fluorescent signals, improved z-resolution, and compatibility with high-throughput applications in microplate formats, and the 60 µm deep-sectioning spinning disk (DSD), which reduces pinhole crosstalk, a phenomenon that results in high background signal when imaging thick biological samples. We will provide a brief overview of the optical challenges that these improvements overcome, then present applications that benefit from their implementation. These new features of the Cytation C10 confocal imaging reader deliver several key advantages and benefits while overcoming critical challenges of confocal imaging.

This presentation will include:

• An introduction to the fully automated water immersion imaging system and deep-sectioning disk available on the Cytation C10
• Techniques for optimizing spinning disk confocal imaging of 3D samples
• Tips for achieving robust quantitative image analysis using application examples
• Guidance on conducting high-throughput imaging studies using the new Cytation C10 features