InfinityLab Poroshell 120 EC-C8
An EC-C8 bonded phase is applied to the totally porous outer layer. It will be less retentive for non-polar samples than InfinityLab Poroshell 120 EC-C18. The EC-C8 bonded phase is made by first chemically bonding a dense monolayer of dimethyl-n-octyl silane stationary phase to the porous shell of the Poroshell 120 silica support. The bonded phase packing is then endcapped using proprietary reagents and procedures to obtain maximum deactivation of the silica surface. The exhaustive endcapping makes it ideal for use with basic compounds, especially those that produce poor peak shapes on other columns. These columns can be used for a wide range of applications.

Particle Size (µm)	Length (mm)	id	Specifications	Method Development Notes	Working with LC/MS
4.0, 2.7, and 1.9 μm superficially porous particles, comprised of solid core and porous outer shell.	30 50 75 100 150	2.1 3.0 4.6	Endcapped Pore size: 120 Å Surface area: 130 m2/g pH: 2.0 - 8.0 Max temperature: 60 °C Max pressure: 1300 bar (1.9 μm), 600 bar (2.7 and 4 μm) Carbon load: 5%	Start with 5% methanol or acetonitrile in water as the initial solvent, and 100% methanol or acetonitrile as the final solvent. We recommend adding 0.1% formic acid in both A and B bottles.	If using LC/MS, we recommend starting with 5 - 10 mM ammonium formate, ammonium acetate, ammonium hydroxide, 0.1% acetic acid or 0.1% formic acid. We recommend against using ammonium bicarbonate.

InfinityLab Poroshell 120 EC-CN
InfinityLab Poroshell 120 EC-CN is made by first chemically bonding a dense monolayer of dimethyl-cyanopropylsilane stationary phase to a the porous shell of Poroshell 120 silica support. The bonded phase is double-endcapped using proprietary reagents and procedures to obtain maximum deactivation of the silica surface. The extra-dense coatng of bonded phase and exhaustive endcapping simultaneously deactivate the silica surface from deleterious interactions with samples and also protects the silica from dissolution in intermediate and higher pH environments.

Particle Size (µm)	Length (mm)	id	Specifications	Method Development Notes	Working with LC/MS
2.7 µm superficially porous particles, comprised of solid core and porous outer shell.	50 100 150	2.1 3.0 4.6	Non-endcapped; Pore size: 120 Å Surface area: 130 m2/g pH: 2.0 - 9.0 Max temperature: 60 °C Max pressure: 600 bar	Start with 5% methanol or acetonitrile in water as the initial solvent, and 100% methanol or acetonitrile as the final solvent. We recommend adding 0.1% formic acid in both A and B bottles.	If using LC/MS, we recommend starting with 5 - 10 mM ammonium formate, ammonium acetate, ammonium hydroxide, 0.1% acetic acid or 0.1% formic acid. We recommend against using ammonium bicarbonate.

InfinityLab Poroshell 120 StableBond SB-C18
The StableBond SB-C18 bonded phase is made by chemically bonding a sterically-protected C18 stationary phase to the porous shell of the Poroshell 120 silica support. The densely covered, sterically protected, diisobutyl-n-octadecylsilane stationary phase is chemically stable and gives long column life at low pH (pH 1- 2). The Agilent InfinityLab Poroshell 120 SB-C18 column is not endcapped, which provides greater alternative selectivity. It can be used for basic, neutral or acidic samples and is particularly well suited for use with aggressive low pH mobile phases (for example, pH <2, high ionic strength (>25 mM), ion-pair additives, etc.) since the steric protection of the bonded phase resists degradation with such mobile phases.

Particle Size (µm)	Length (mm)	id	Specifications	Method Development Notes	Working with LC/MS
2.7 µm superficially porous particles, comprised of solid core and porous outer shell.	50 100 150	2.1 3.0 4.6	Non-endcapped Pore size: 120 Å Surface area: 130 m2/g pH: 1.0 - 8.0 Max temperature: 60 °C Max pressure: 600 bar Carbon load: 7.5%	Start with 5% methanol or acetonitrile in water as the initial solvent, and 100% methanol or acetonitrile as the final solvent. We recommend adding 0.1% formic acid in both A and B bottles.	If using LC/MS, we recommend starting with 5 - 10 mM ammonium formate, ammonium acetate, ammonium hydroxide, 0.1% acetic acid or 0.1% formic acid. We recommend against using ammonium bicarbonate.

InfinityLab Poroshell 120 StableBond SB-C8
The StableBond SB-8 bonded phase is made by chemically bonding a sterically-protected C8 stationary phase to the porous shell of the Poroshell 120 silica support. The densely-covered, sterically-protected diisopropyl-n-octylsilane stationary phase is chemically stable and gives longer column life. The Agilent InfinityLab Poroshell 120 SB-C8 column is non-endcapped for greater alternative selectivity. It can be used for basic, neutral or acidic samples, and is particularly well suited for use with aggressive low pH mobile phases (for example, pH <2, high ionic strength (> 25 mM), ion-pair additives, etc.) since the steric protection of the bonded phase resists degradation with such mobile phases. This phase used in many established USP methods, and enables method transfer from existing ZORBAX SB-C8 columns in these methods.

Particle Size (µm)	Length (mm)	id	Specifications	Method Development Notes	Working with LC/MS
2.7 µm superficially porous particles, comprised of solid core and porous outer shell.	30 50 75 100 150	2.1 3.0 4.6	Non-endcapped; Pore size: 120 Å Surface area: 130 m2/g pH: 1.0 - 8.0 Max temperature: 80 °C Max pressure: 600 bar Carbon load: 4.5%	Start with 5% methanol or acetonitrile in water as the initial solvent, and 100% methanol or acetonitrile as the final solvent. We recommend adding 0.1% formic acid in both A and B bottles.	If using LC/MS, we recommend starting with 5 - 10 mM ammonium formate, ammonium acetate, ammonium hydroxide, 0.1% acetic acid or 0.1% formic acid. We recommend against using ammonium bicarbonate.

InfinityLab Poroshell 120 Phenyl-Hexyl
The InfinityLab Poroshell 120 Phenyl-Hexyl column combines the productivity enhancements of superficially porous particle technology with Phenyl-Hexyl bonding, for alternate selectivity to C18 and C8 phases. The Phenyl-Hexyl packing is made by first chemically bonding a dense monolayer of dimethylphenylhexylsilane stationary phase to the porous outer layer. The bonded phase is then doubly endapped using proprietary reagents and procedures to obtain maximum deactivation. These columns can be used for acidic and neutral samples, but are especially suited for separating basic compunds that produce poor peak shapes on other columns.

Size (µm)	Length (mm)	id	Specifications	Method Development Notes	Working with LC/MS
4.0, 2.7, and 1.9 μm superficially porous particles, comprised of solid core and porous outer shell.	50 100 150	2.1 3.0 4.6	Endcapped Pore size: 120 Å Surface area: 130 m2/g pH: 2.0 - 8.0 Max temperature: 60 °C Max pressure: 1300 bar (1.9 μm), 600 bar (2.7 and 4 μm) Carbon load: 8%	Start with 5% methanol or acetonitrile in water as the initial solvent, and 100% methanol or acetonitrile as the final solvent. We recommend adding 0.1% formic acid in both A and B bottles.	If using LC/MS, we recommend starting with 5 - 10 mM ammonium formate, ammonium acetate, ammonium hydroxide, 0.1% acetic acid or 0.1% formic acid. We recommend against using ammonium bicarbonate.

InfinityLab Poroshell 120 StableBond SB-Aq
The Agilent InfinityLab Poroshell 120 SB-Aq column combines the productivity enhancements of superficially porous particle technology with unique SB-Aq selectivity. InfinityLab Poroshell 120 SB-Aq is non-endcapped, for greater alternative selectivity. This proprietary phase is ideal for polar compounds and can be used with 100% water.

Size (µm)	Length (mm)	id	Specifications	Method Development Notes	Working with LC/MS
2.7 µm superficially porous particles, comprised of solid core and porous outer shell.	50 100 150	2.1 3.0 4.6	Non-endcapped Pore size: 120 Å Surface area: 130 m2/g pH: 1.0 - 8.0 Max temperature: 80 °C Max pressure: 600 bar Carbon load: proprietary	Start with 5% methanol or acetonitrile in water as the initial solvent, and 100% methanol or acetonitrile as the final solvent. We recommend adding 0.1% formic acid in both A and B bottles. Poroshell SB-Aq can be used with 100% aqueous.	If using LC/MS, we recommend starting with 5 - 10 mM ammonium formate, ammonium acetate, ammonium hydroxide, 0.1% acetic acid or 0.1% formic acid. We recommend against using ammonium bicarbonate.

InfinityLab Poroshell 120 Bonus-RP
The Agilent InfinityLab Poroshell 120 Bonus-RP combines the productivity enhancements of superficially porous particle technology with unique Bonus-RP selectivity. This alkyl-amide produces orthoganol results for a number of separations, and can be helpful for resolving difficult compounds.

Size (µm)	Length (mm)	id	Specifications	Method Development Notes	Working with LC/MS
2.7 µm superficially porous particles, comprised of solid core and porous outer shell.	50 100 150	2.1 3.0 4.6	Endcapped (triple) Pore size: 120 Å Surface area: 130 m2/g pH: 2.0 - 9.0 Max temperature: 60 °C Max pressure: 600 bar Carbon load: 7.5%	Start with 5% methanol or acetonitrile in water as the initial solvent, and 100% methanol or acetonitrile as the final solvent. We recommend adding 0.1% formic acid in both A and B bottles.	If using LC/MS, we recommend starting with 5 - 10 mM ammonium formate, ammonium acetate, ammonium hydroxide, 0.1% acetic acid or 0.1% formic acid. We recommend against using ammonium bicarbonate.

InfinityLab Poroshell 120 PFP
The Agilent InfinityLab Poroshell 120 PFP provides another unique phase chemistry on superficially porous particles. The pentafluorophenyl (PFP) end group provides excellent separation of polar and halogenated analytes, as well as powerful selectivity towards aromatic groups due to π-electron interactions.

Size (µm)	Length (mm)	id	Specifications	Method Development Notes	Working with LC/MS
4.0, 2.7, and 1.9 μm superficially porous particles, comprised of solid core and porous outer shell.	50 100 150	2.1 3.0 4.6	Endcapped Pore size: 120 Å Surface area: 130 m2/g pH: 2.0 - 9.0 Max temperature: 60 °C Max pressure: 1300 bar (1.9 μm), 600 bar (2.7 and 4 μm)	Start with 5% methanol or acetonitrile in water as the initial solvent, and 100% methanol or acetonitrile as the final solvent. We recommend adding 0.1% formic acid in both A and B bottles.	If using LC/MS, we recommend starting with 5 - 10 mM ammonium formate, ammonium acetate, ammonium hydroxide, 0.1% acetic acid or 0.1% formic acid. We recommend against using ammonium bicarbonate.

InfinityLab Poroshell HPH-C18
The InfinityLab Poroshell-C18 offers high efficiency and long life for analyses taking place at elevated pH. A unique, organically modified hybrid silica particle prevents the degradation of the silica surface at alkaline conditions, while preserving the selectivity of the C18 bonded phase.

Size (µm)	Length (mm)	id	Specifications	Method Development Notes	Working with LC/MS
4.0, 2.7, and 1.9 μm superficially porous particles, comprised of solid core and porous outer shell.	50 100 150	2.1 3.0 4.6	Endcapped Pore size: 100 Å Surface area: 130 m2/g pH: 2.0 - 11.0 (buffer dependent, see Notes) Max temperature: 60 °C Max pressure: 1300 bar (1.9 μm), 600 bar (2.7 and 4 μm)	Start with 5% methanol or acetonitrile in water as the initial solvent, and 100% methanol or acetonitrile as the final solvent. We recommend adding 0.1% formic acid in both A and B bottles. For high pH applications, use 10 - 20 mM buffers such as phosphate or borate buffer (pH 8 - 9), organic buffers (pH ~8 - 11), ammonium acetate (pH 8 -10).	If using LC/MS, we recommend starting with 5 - 10 mM ammonium formate, ammonium acetate, ammonium hydroxide, 0.1% acetic acid or 0.1% formic acid. We recommend against using ammonium bicarbonate as a first choice.

InfinityLab Poroshell HPH-C8
The InfinityLab Poroshell HPH-C8 offers high efficiency and long life for analyses taking place at elevated pH. A unique, organically modified hybrid silica particle prevents the degradation of the silica surface at alkaline conditions, while preserving the selectivity of the C8 bonded phase.

Size (µm)	Length (mm)	id	Specifications	Method Development Notes	Working with LC/MS
2.7 µm superficially porous particles, comprised of solid core and porous outer shell.	50 100 150	2.1 3.0 4.6	Endcapped Pore size: 100 Å Surface area: 130 m2/g pH: 2.0 - 11.0 (buffer dependent, see Notes) Max temperature: 60 °C Max pressure: 600 bar	Start with 5% methanol or acetonitrile in water as the initial solvent, and 100% methanol or acetonitrile as the final solvent. We recommend adding 0.1% formic acid in both A and B bottles. For high pH applications, use 10 - 20 mM buffers such as phosphate or borate buffer (pH 8 - 9), organic buffers (pH ~8 - 11), ammonium acetate (pH 8 -10).	If using LC/MS, we recommend starting with 5 - 10 mM ammonium formate, ammonium acetate, ammonium hydroxide, 0.1% acetic acid or 0.1% formic acid. We recommend against using ammonium bicarbonate as a first choice.

InfinityLab Poroshell HILIC-Z
The HILIC-Z phase is a novel zwitterionic chemistry bonded to a hybrid Poroshell silica particle. The zwitterionic chemistry optimizes interactions between highly charged polar compounds and the stationary phase, resulting in improved retention, peak shape, and resolution of polar compounds. This unique bonding and particle combination results in a chemistry with extended temperature and pH robustness for applicability to a wide range of challenging polar compound analysis applications.

Size (µm)	Length (mm)	id	Specifications	Method Development Notes	Working with LC/MS
2.7 µm superficially porous particles, comprised of solid core and porous outer shell.	50 100 150	2.1 3.0 4.6	Pore size: 120 Å Surface area: 130 m2/g pH: 3.0 - 11.0 Max temperature: 80 °C Max pressure: 600 bar (2.7 µm) Carbon load: proprietary	Equilibrate with 20% aqueous with 5 column volumes, then switch to starting mobile phase conditions and equilibrate for 10 column volumes. Start with 5-10% aqueous then run gradient to 50% aqueous/acetonitrile with 10mM salt buffer as the final mobile phase buffer concentration. Use Ammonium formate or acetate buffers.	Same as Method Development

InfinityLab Poroshell HILIC-OH5
The HILIC-OH5 phase is a poly-hydroxy fructan chemistry covalently bonded to Poroshell silica particles. This chemistry offers a very different selectivity to the zwitterionic and bare silica HILIC phases. The bonded fructan phase offers greater stability and reproducibility for HILIC separations compared to bare-silica HILIC columns.

Size (µm)	Length (mm)	id	Specifications	Method Development Notes	Working with LC/MS
2.7 µm superficially porous particles, comprised of solid core and porous outer shell.	50 100 150	2.1 4.6	Pore size: 120 Å Surface area: 130 m2/g pH: 1.0 - 7.0 Max temperature: 45 °C Max pressure: 400 bar (2.7 µm) Carbon load: proprietary	Equilibrate with 20% aqueous with 5 column volumes, then switch to starting mobile phase conditions and equilibrate for 10 column volumes. Start with 5-10% aqueous then run gradient to 50% aqueous/acetonitrile with 10mM salt buffer as the final mobile phase buffer concentration. Use Ammonium formate or acetate buffers.	Same as Method Development

InfinityLab Poroshell 120 HILIC
InfinityLab Poroshell 120 HILIC columns are non-bonded silica columns based on high-performance Poroshell 120 superficially porous silica that has a solid silica core and a porous outer layer. This type of particle provides high efficiency at lower pressures when compared to small, totally porous particles and is ideal for fast or high-resolution separations of many types of analytes. Agilent Poroshell 120 HILIC columns are for use in hydrophilic interaction chromatography (HILIC) applications, typically used for retention and resolution of small polar compounds.

Particle Size (µm)	Length (mm)	id	Specifications	Method Development Notes	Working with LC/MS
4.0, 2.7, and 1.9 μm superficially porous particles, comprised of solid core and porous outer shell.	50 100 150	2.1 3.0 4.6	Non-endcapped; Pore size: 120 Å Surface area: 130 m2/g pH: 0 - 8.0 Max pressure: 1300 bar (1.9 μm), 600 bar (2.7 and 4 μm)	Equilibrate with 20% aqueous with 5 column volumes, then switch to starting mobile phase conditions and equilibrate for 10 column volumes. Start with 5-10% aqueous then run gradient to 50% aqueous/acetonitrile with 10mM salt buffer as the final mobile phase buffer concentration. Use Ammonium formate or acetate buffers.	Same as Method Development

InfinityLab Poroshell Chiral-T
The Poroshell Chiral-T phase is a macrocyclic glycopeptide (teicoplanin) bonded to superficially porous Poroshell 120 particles. This complex bonded phase chemistry gives unique selectivity for chiral separations as a result of a variety of chiral recognition sites on the teicoplanin molecule. Alternate selectivity is seen between teicoplanin and vancomycin phases. Improved interactions result in better peak shapes, and faster, more efficient chiral separations are achieved using superficially porous particles, compared to traditional chiral columns that use totally porous particles.

Size (µm)	Length (mm)	id	Specifications	Method Development Notes	Working with LC/MS
2.7 µm superficially porous particles, comprised of solid core and porous outer shell.	50 100 150	2.1 4.6	Pore size: 120 Å Surface area: 130 m2/g pH: 2.5 - 7.0 Max temperature: 45 °C Max pressure: 400 bar (2.7 µm) Carbon load: proprietary	Screen with isocratic conditions: Polar ionic: 100/0.2 wt% MeOH/ammonium formate Polar organic: 60/40/0.3/0.2 ACN/MeOH/acetic acid/TEA Reversed-phase: 30/70 MeOH/20 mM ammonium formate (pH 4) Normal phase: 20/80/0.3/0.2 EtOH/heptane/acetic acid/TEA	Use polar ionic, polar organic, or reversed-phase conditions as noted.

InfinityLab Poroshell Chiral-V
The Poroshell Chiral-V phase is a macrocyclic glycopeptide (vancomycin) bonded to superficially porous Poroshell 120 particles. This complex bonded phase chemistry gives unique selectivity for chiral separations as a result of a variety of chiral recognition sites on the vancomycin molecule. Alternate selectivity is seen between teicoplanin and vancomycin phases. Improved interactions results in better peak shapes, and faster, more efficient chiral separations are achieved using superficially porous particles, compared to traditional chiral columns that use totally porous particles.

Size (µm)	Length (mm)	id	Specifications	Method Development Notes	Working with LC/MS
2.7 µm superficially porous particles, comprised of solid core and porous outer shell.	50 100 150	2.1 4.6	Pore size: 120 Å Surface area: 130 m2/g pH: 2.5 - 7.0 Max temperature: 45 °C Max pressure: 400 bar (2.7 µm) Carbon load: proprietary	Screen with isocratic conditions: Polar ionic: 100/0.2 wt% MeOH/ammonium formate Polar organic: 60/40/0.3/0.2 ACN/MeOH/acetic acid/TEA Reversed-phase: 30/70 MeOH/20 mM ammonium formate (pH 4) Normal-phase: 20/80/0.3/0.2 EtOH/heptane/acetic acid/TEA	Use polar ionic, polar organic, or reversed-phase conditions as noted.

InfinityLab Poroshell Chiral-CD
The Poroshell Chiral-CD phase is a hydroxypropylated-beta-cyclodextrin chemistry bonded to superficially porous Poroshell 120 particles. This unique chemistry offers different types of interactions between chiral enantiomers and the stationary phase compared to macrocyclic glycopeptides or cyclofructan phases, resulting in different selectivity. Improved interactions between enantiomers and stationary phases result in improved peak shapes. Faster, more efficient chiral separations are achieved using superficially porous particles, compared to traditional chiral columns that use totally porous particles.

Size (µm)	Length (mm)	id	Specifications	Method Development Notes	Working with LC/MS
2.7 µm superficially porous particles, comprised of solid core and porous outer shell.	50 100 150	2.1 4.6	Pore size: 120 Å Surface area: 130 m2/g pH: 3.0 - 7.0 Max temperature: 45 °C Max pressure: 400 bar (2.7 µm) Carbon load: proprietary	Screen with isocratic conditions: Reversed-phase: 30/70 ACN/20 mM Ammonium formate buffer (pH 4) Polar organic: 60/40/0.3/0.2 ACN/MeOH/acetic acid/TEA	Use polar organic or reversed-phase conditions as noted.

InfinityLab Poroshell Chiral-CF
The Poroshell Chiral-CF phase is a derivatized cylcofructan (CF6) chemistry covalently bonded to superficially porous Poroshell 120 particles. This chemistry is ideal for the analysis of primary amines and can be used in normal phase and reversed phase modes. Faster, more efficient chiral separations are achieved using superficially porous particles, compared to traditional chiral columns that use totally porous particles.

Size (µm)	Length (mm)	id	Specifications	Method Development Notes	Working with LC/MS
2.7 µm superficially porous particles, comprised of solid core and porous outer shell.	50 100 150	2.1 4.6	Pore size: 120Å Surface area: 130 m2/g pH: 3.0 - 7.0 Max temperature: 45 °C Max pressure: 400 bar (2.7 µm) Carbon load: proprietary	Screen with isocratic conditions: Polar organic: 60/40/0.3/0.2 ACN/MeOH/acetic acid/TEA Normal-phase: 20/80/0.3/0.2 EtOH/heptane/acetic acid/TEA	Start with conditions as noted in Method Development.