Answer: Yes, but note that there are severe limitations for using Splitless mode with the headspace sampler.

Background: Typically a Splitless injection is intended to cold trap the sample in a small band on the head of the capillary column from a liquid injection in the inlet. In that case the sample does not have far to travel and low flows are okay to get the sample on the column in a reasonable length of time and in a small band on the column.

With a Splitless mode injection on the headspace there is a significant volume of tubing for the sample to go through before it reaches the inlet. With the low flows that are typical for a Splitless injection it takes a very long time for the sample to get on the column. Because of this you will encounter two problems:

1. Severe band broadening of the sample on the column. (Broad peaks)
2. Loss of active compounds due to the length of time the sample is in contact with heated pneumatic parts.

Note: Cryo-focusing or sub-ambient oven temperatures may help these issues.

Another consideration is the Split/Splitless inlet may be unstable due the low flows and greatly increased volume added to the system by the headspace pneumatics. Splitless mode can be used when the column flow is 20 to 30 ml/min with a 530um column. Also the 6890 GC needs to have Auto Prep Run enabled in order to have the sequence run correctly. To do that you must enable it from the front panel of the 6890 GC. To enable the function:

1. On the front panel of the 6890 GC press the Config key.
2. Scroll the display down to Instrument and press the Enter key.
3. At Auto prep run on the display press the On key to enable the feature.