Agilent HPLC-Chip Sensitivity: Sulfadomethoxine in Serum

Sulfadimethoxine was analyzed with the HPLC-Chip/MS system and LC/MSD Trap XCT mass spectrometer. A 1 µL sample was injected on the enrichment column and forward flushed to the analytical column for separation and detection by the MS. An LOD of 10 fg (32 attomoles) was estimated from the serum blank.

Agilent HPLC-Chip Sensitivity:
Quantitative Proteomics

For accurate high-throughput absolute quantitation, the Agilent 6410 Triple Quad MS/MS provides targeted MRM peptide quantitation at femtogram detection levels as well as the necessary throughput and 24/7 day-after-day reliability. The external quantitation curve of peroxidase peptide DTIVNELR from 10 amol to
10 fmol shows excellent linearity in a complex matrix such as serum.