Applications

Identification of Contaminants

Identification of contaminants on a PLgel 10 µm Preparative column

This phenol product contained small amounts of two contaminant species. By using very high loading, sufficient quantities of fractions 1 and 3 were collected to permit identification by infra-red spectroscopy.

Key
1 & 3 Contaminants
2 Phenol

Column:

PLgel 10 µm 100Å , 2 x 300 x 25.0 mm

Eluent:

Acetone

Flow Rate:

10.0 mL/min

Loading:

100 mg/mL (200 g on column)

Detector:

RI


Product Deformulation

Product Deformulation on a PLgel 10 µm Preparative column


Loading in preparative GPC is molecular weight dependent and is much greater for low MW materials than for polymers. Even for polymers, a minimum of 10x scale up is routine but for very low molecular weight materials, the loading can be significantly increased.

Column:

PLgel 10 µm 100Å , 2 x 300 x 25.0 mm

Eluent:

THF

Flow Rate:

9.0 mL/min

Loading:

1. 5 mg/mL, 1 mL (5 mg on column)
2. 100 mg/mL, 3 mL (300 mg on column)

Detector:

RI


Fractionation

Fractionation analysis on a PLgel 10 µm Preparative columns


Each fraction of this phenanthrene-formaldehyde resin was collected using a 'heart-cut' technique to avoid cross-fraction contamination. Identification was carried out using mass spectroscopy and infra-red spectroscopy.

Column:

PLgel 10 µm 500Å , 2 x 300 x 25.0 mm

Eluent:

Dichloromethane

Flow Rate:

9.0 mL/min

Loading:

100 mg/mL, 2 mL (200 g on column)

Detector:

UV, 254 nm