| Application |
PLRP-S Media Pore Size |
|
100Å |
300Å |
1000Å |
4000Å |
| Synthetic biomolecules, peptides, and oligonucleotides |
✓ |
✓ |
|
|
| Recombinant biomolecules, peptides, and proteins |
✓ |
✓ |
|
|
| Large biomolecules, antibodies, DNA fragments |
|
|
✓ |
✓ |
| Small molecules, unstable compounds including metal sensitivity |
✓ |
|
|
|
| Column: |
PLRP-S 100Å, 4.6 x 50 mm, 3 µm |
| Mobile Phase: |
A: 100mM Triethylammonium acetate (TEAA) |
|
B: 100 mM TEAA in 25:75 Acetonitrile:water |
| Flow Rate: |
1 mL/min |
| Gradient: |
25% B 0 min, 35% B in 2 min, 45% B 22.5 min, 45% B 23 min, 25% B 23.05 min, 25% B 26 min |
| Temperature: |
80 ºC |
| Column: |
PLRP-S 100Å, 4.6 x 250 mm, 10 µm |
| Sample: |
30 µL containing 1.5 mg of crude peptide |
| Mobile Phase: |
0.1% TFA in 21% ACN: 79% water |
| Flow Rate: |
1 mL/min (360 cm/hr) |
| HPLC analysis of the fractions collected across the peak showed that fractions 1 to 4 contained only the peptide of interest and that the level of the critical impurity increased with increasing fraction number. Using the high efficiency PLRP-S column it was possible to obtain from the crude, 91.7% pure, a recovery of 97% with 100% purity. |
|