Presented by Kristaps Klavins, Deputy Head of the Proteomics and Metabolomics Facility, Research Center for Molecular Medicine of the Austrian Academy of Sciences.


The central carbon metabolism including glycolysis, the pentose phosphate pathway, and the TCA-cycle is essential for all biological systems. The measurements of metabolites involved in these pathways, known as metabolomics, can provide valuable insights about cell biology for various life science applications including biomedical research. However, the chromatographic separation of these, mostly polar compounds (e.g. amino acids, carboxylic acids, cofactors, nucleotides, sugars, and sugar-phosphates), is still a challenging task. It has been shown that ion pair- reverse phase chromatographic separation is a suitable technique to tackle this challenge. We took advantage of the Agilent metabolomics dynamic MRM database and method to establish a robust workflow for the quantification of almost 200 metabolites.

For Research Use Only. Not for use in diagnostic procedures.

Learn more about the Agilent Metabolomics dMRM Database and Method solution.