Agilent Seahorse XF analyzers allow you to sequentially inject up to four compounds of various types, including inhibitors, stimulators or substrates into each well during the course of an experiment.
The ability to add multiple drugs makes it possible to perform a wide variety of assays including ATP Production Rate Assay, T Cell Metabolic Profiling Assays, Cell Mito Stress Test, Substrate Oxidation Stress Tests, Fatty Acid Oxidation Assay, and Glycolytic Rate Assay as well as assays of your own design.