Agilent 1100 Series HPLC Maintenance
LC/MSD / Repairing the LC/MSD / Adjusting the Electrospray Nebulizer Needle

When required
After replacing the electrospray nebulizer needle or if symptoms indicate the needle is not correctly adjusted. 
Tools required
Adjustment fixture G1946-20215
Gloves, clean
Magnifier, G1946-80049
Wrench 3-mm, open-end, 8710-2699
Wrench ¼-inch x 5/16-inch, open-end, 8710-0510
Parts required
None 

  1. Install the nebulizer in the adjustment fixture. 
  2. Loosen the needle holder locknut.
  3. Position the magnifier so you can see the tip of the nebulizer. 
  4. Adjust the needle holder until the needle extends slightly less than one-half its own diameter beyond the tip of the nebulizer. 
  5. Tighten the locknut.  Make sure this does not change the position of the needle.
  6. Remove the nebulizer from the adjustment fixture and reinstall it in the electrospray spray chamber.
  7. Be very careful not to hit the tip of the nebulizer against anything. With the needle extended, it is especially vulnerable. Any damage will have a large, negative effect on system performance.
  8. Record this procedure in the Maintenance Logbook in the Diagnosis view of the system software.