During method development in ion-exchange chromatography, buffer systems are used to create pH or salt gradients for pH scouting. In combination with the Agilent 1260 Infinity Bio-inert LC System, Agilent Buffer Advisor software helps you to automate the blending of these buffer systems, eliminating the tedious and error-prone steps associated with manual buffer mixing. Learn more about the basic principles of buffers in ion-exchange chromatography.