Pathological mechanisms which underlie chronic diseases afflicting developed nations, converge on the metabolic pathways that control energy production in the cell. The availability of low-cost, high-calorie foods, and a contemporary, sedentary...

Mitochondrial functions in bioenergetics and intermediary metabolism are often viewed separately from their role in apoptotic signaling. However, the effect of different bioenergetic pathways on apoptosis has not been carefully examined. Using the...

Glycolysis is involved in a wide array of normal physiological processes [stem cells pluripotency, acquired immune response, muscle function, brain function], as well as pathophysiological processes [tumorigenesis]. Glycolysis consists of a series...

A particularly useful and straight-forward protocol has been to add in series an inhibitor of the ATP synthase (oligomycin), an uncoupler (FCCP) and a respiratory chain inhibitor (antimycin or rotenone) to obtain mitochondrial profile of cells under...

During oxidative phosphorylation, oxidation of substrates is coupled to phosphorylation of ADP to ATP. Mitochondria pump out protons to build up a protonmotive force, which is then used to drive protons back through the ATP synthase, generating ATP....

To investigate the effects of hypoxia in cultured cells, XF technology has been adapted to function in reduced O2 environments (i.e. a hypoxia chamber). Use of a hypoxia chamber in conjunction with a newly designed software application for XF...

Recent discoveries have linked mitochondrial dysfunction and defects in cellular bioenergetics to the pathophysiology of many diseases including obesity, diabetes, cancer, cardiomyopathy and neurodegeneration. The introduction of Extracellular Flux...

The Seahorse XF24 Analyzer instrument was originally designed for use with intact cells attached to a tissue culture plate. However, as many researchers routinely utilize isolated mitochondria to answer fundamental questions about mitochondrial...

Plasma membrane permeabilization allows control of substrate provision, circumvents the need for mitochondrial isolation, and is particularly useful when studying genetically modified cells, or when limited biological sample is available. Multiple...

Find upcoming Agilent Cell Analysis Technology webinars and view recordings of past webinars on key topics in cell metabolism research and bioenergetics.

XF Extracellular Flux technology is commonly used to measure cellular bioenergetics, and determine the role of metabolism in regulating cell phenotype. From measuring basic intracellular mitochondrial function, XF analysis has now evolved to...

An on-demand webinar titled "Using Agilent Seahorse XF Technology to Study Energy Metabolism in Mycobacterium tuberculosis"