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Describes a procedure for decontaminating Seahorse XF analyzers using vaporized hydrogen peroxide (VHP)
Demonstration of a dose response study of mitochondrial inhibitors being carried out using the Agilent Seahorse XFe Analzyer and the Bravo Automated Liquid Handling Platform.
Seahorse XFe96 Analyzers measure OCR and ECAR of live cells in a 96-well plate format. OCR and ECAR rates are key indicators of mitochondrial respiration and glycolysis and these measurements provide a systems-level view of cellular metabolic...
Learn more about our entire cell analysis portfolio and identify the best solution for your research needs.
Learn more about how XF Technology Works
For Research Use Only. Not for use in diagnostic procedures.
Comprehensive PC system requirements and other information for proper operation of Seahorse software for instrument control and data analysis.
Measuring white adipose tissue (WAT) mitochondrial function reveals impaired fatty acid stimulated uncoupling from obese individuals.
Bravo is used to automate three liquid handling steps for Seahorse XFe96 assay preparation: washing cells, preparing injection solutions, sensor cartridge loading.
Application Note - Normalization of Agilent Seahorse XF data by in-situ cell counting using a BioTek Cytation 5
This manual contains information for operating and maintaining the Agilent Seahorse XFe Analyzer, including both the 24-well and 96-well models running Wave software
Characterize metabolic functionality of adipocytes differentiated from the pre-adipocyte 3Y3-L1 cell culture line using the Agilent Seahorse XF/XFe Analyzer.
This Application Note shows how Seahorse XF technology can be used to determine specific metabolic states associated with cellular dysfunction and disease
Wave 2.4 software Release Notes
Agilent Seahorse XF analyzers utilize a patented transient microchamber which enables sensitive, precise, and nondestructive metabolic measurements in real time.
Mitochondrial function correlates to brown adipocyte function
Agilent Seahorse XF analyzers incorporate integrated injection ports that sequentially deliver up to 4 compounds allowing dose response, agonist or antagonist response, or pathway perturbation analysis of each sample.