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1 - 20 of 53 results
AI-powered, label-free cell segmentation in RTCA eSight enables robust brightfield cell counting and fluorescence-assisted classification across diverse assays,improving throughput and reproducibility.
The protocol outlines basic steps for immune cell killing assays using Agilent xCELLigence RTCA systems, including RTCA SP, MP, and eSight systems.
In this study, the EpCAM CAR T cells were challenged with an EpCAM-expressing breast cancer cell line, T47D cells, over seven consecutive rounds. During each challenge, the cytolytic activity of CAR T was measured and evaluated in real time using an...
Application note featuring developed 3D multispheroid assays using xCELLigence RTCA eSight to monitor tumor-like structures for accurate drug and immunotherapy testing in a scaffold based model.
The Agilent xCELLigence real-time cell analysis (RTCA) eSight system gives its users the flexibility to use both label-free impedance and/or imaging in four channels for the real-time monitoring of cell proliferation and cytotoxicity. The eSight has...
Here we describe image-based immune cell killing assays of 3D single tumor spheroids expressing RFP using the xCELLigence RTCA eSight.
This application note demonstrates a robust method for performing real-time antibody internalization assays using the Agilent xCELLigence RTCA eSight combined with CypHer5E pH-sensitive dyes.
This document is an information guide and checklist prepared for you that outlines the supplies, space, and utility requirements for the system set up in your lab.
In this application note, we demonstrate the utility of a cell-by-cell analysis module that uses brightfield imaging to enable label-free cell segmentation and classification based on area, eccentricity, or fluorescence intensity, allowing for...
In this technical overview, we embedded a single HT-1080 tumor spheroid into extracellular matrix, using the 3D spheroid invasion module of the Agilent xCELLigence RTCA eSight system to continuously acquire brightfield and fluorescent channel images...
Using real-time cell potency and bioenergetic assays to optimize critical process parameters for manufacturing of engineered T cells.
Using real-time cell potency and bioenergetic assays to optimize critical process parameters for manufacturing of engineered T cells with eSight and Seahorse technology.
This application note demonstrates the signal recording of the xCELLigence RTCA eSight, enabling visualization and quantification of cell migration and invasion and the speed of wound closure healing.
This application note describes a novel real-time co-culture assay using RTCA eSight for interrogating NK92 immune cell invasion and cytotoxicity and migratory kinetic profiles based on ECM and effects of TME.
Technical overview describes a method for monoclonal cell isolation using a live cell analysis system, the Agilent xCELLigence RTCA eSight, with a limiting dilution cloning method.
This application note evaluates the Agilent xCELLigence RTCA eSight as a tool to monitor BiTE antibody-mediated cancer cell lysis.
This application note describes pulse labeling methods based on inorganic quantum dots, organic nanoparticles, dyes that covalently modify cytoplasmic proteins, and baculovirus. After evaluating each reagent’s labeling efficiency across a population...
This tech note demonstrates the ability of the RTCA eSight assay to identify and characterize antiviral drugs using adenovirus as a model system in real time.
Measure and quantify enhanced immune cell cancer killing with antibody drug treatment; shown here is a combo of trastuzumab and PBMCs targeting HER-2 positive cells
The Agilent xCELLigence RTCA eSight monitors 3D tumor spheroid growth/shrinkage in real time using brightfield plus red, green, and blue fluorescence.