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SCS110 Competent Cells

SCS110 competent cells for generating unmethylated DNA improve quality and yield of miniprep DNA.

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SCS110 competent cells for generating unmethylated DNA are also an EndA- variant, which greatly improves the yield and quality of plasmid miniprep DNA. |SCS110 is an endA– derivative of the JM110 strain. The SCS110 strain is ideal for preparing plasmid or phagemid DNA free of Dam or Dcm methylation so that the DNA can be restriction digested by one or more methylation sensitive restriction enzymes. SCS110 is deficient for two methylases (Dam and Dcm) found in most strains of E. coli. Dam methylase recognizes the DNA sequence GATC and methylates the adenine residue at the N-6 position, while Dcm methylase recognizes the DNA sequence CCAGG and CCTGG and methylates the internal cytosine at the C-5 position. SCS110 cells are endonuclease (endA) deficient, greatly improving the quality of miniprep DNA. SCS110 cells contain the lacIqZΔM15 gene on the F episome, allowing bluewhite screening for recombinant plasmids.

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200247

SCS110 Competent Cells. EndA- for improved yield and quality of miniprep DNA.

  • Unit:

    5

  • Regulatory Status:

    RUO

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Brochures
Competent Cells: Difficult Cloning, Protein Expression, and General Cloning Comp Cells (PDF)

Competent Cells: Difficult Cloning, Protein Expression, and General Cloning Comp Cells

  • Publication Part Number: 5989-8281EN
  • Created: 01 Sep 2024
  • 2 MB
  • PDF
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Brochures
Molecular and Synthetic Biology Solutions (PDF)

Reliable next-generation mutagenesis and cloning tools

  • Publication Part Number: 5991-9163EN
  • Created: 22 June 2023
  • 3 MB
  • PDF
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Posters
Poster_Choose Your Competent Cells With Confidence (PDF)

Competent cells offered for various applications including: difficult cloning (large or ligated DNA, genomic DNA), general cloning (unmethylated DNA, random mutagenesis), and protein expression (solubility & codon bias, phosphorylated proteins).

  • Publication Part Number: 5990-5645EN
  • Created: 28 May 2019
  • 694 KB
  • PDF
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JM110 Competent Cells

Competent cells used to prepare plasmid or phagemid cells for more accurate analysis

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Most E. coli hosts contain both DNA adenine methylation (dam) and DNA cytosine methylation (dcm) genes. These genes code for proteins that methylate specific sequences when DNA is propagated, making subsequent digestion with methylation-sensitive restriction enzymes impossible. The SCS110 strain, lacks both dam and dcm activity. DNA propagated in this strain can be digested by methylation-sensitive enzymes such as Xba I, Cla I and EcoR II.|The JM110 strain is useful for preparing plasmid or phagemid DNA that is free of Dam or Dcm methylation so that the DNA can be restriction digested by one or more methylation-sensitive restriction enzymes. JM110 is deficient for two methylases (Dam and Dcm) found in most strains of E. coli. Dam methylase recognizes the DNA sequence GATC and methylates the adenine residue at the N-6 position, while Dcm methylase recognizes the DNA sequence CCAGG and CCTGG and methylates the internal cytosine at the C-5 position. JM110 cells contain the lacIqZΔM15 gene on the F episome, allowing blue-white screening for recombinant plasmids.
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Safety Data Sheets
SCS110 Competent Cells (PDF)
  • Released: 24 Mar 2025
  • 225 KB
  • PDF
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User Manuals
Gigapack III Gold Packaging Extract, Gigapack III Plus Packaging Extract, and Gigapack III XL Packaging Extract Instruction Manual (PDF)

The single-tube format of Gigapack III packaging extract simplifies the packaging procedure and increases the efficiency and representation of libraries constructed from highl

  • Publication Part Number: 200201-12
  • Created: 06 Oct 2025
  • 223 KB
  • PDF
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User Manuals
QuikChange Lightning Site-Directed Mutagenesis Kit (PDF)

Instructions for the QuikChange Lightning Site-Directed Mutagenesis Kit with detailed requirements, protocols, and transformation guides.

  • Publication Part Number: 210518-12
  • Created: 08 Sep 2023
  • 367 KB
  • PDF
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User Manuals
QuikChange Lightning Multi Site-Directed Mutagenesis Kit (commercial) (PDF)

Instruction manual for the commercial version of QuikChange Lightning Multi Site-Directed Mutagenesis Kit with introduction, guidelines and protocol requirements.

  • Publication Part Number: 210514-12
  • Created: 08 Sep 2023
  • 509 KB
  • PDF
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User Manuals
QuikChange Multi Site-Directed Mutagenesis Kit (Academic) (PDF)

Academic, QuikChange multi mutagenesis control, mutagenic primer design, mutant strand synthesis reaction, Dpn I digestion, transformation guidelines, and troubleshooting.

  • Publication Part Number: 200514-12
  • Created: 08 Sep 2023
  • 483 KB
  • PDF
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User Manuals
QuikChange Multi Site-Directed Mutagenesis Kit (Commercial) (PDF)

Commercial: QuikChange multi mutagenesis control, mutagenic primer design, mutant strand synthesis reaction, Dpn I digestion, transformation guidelines, and troubleshooting.

  • Publication Part Number: 200513-12
  • Created: 08 Sep 2023
  • 478 KB
  • PDF
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User Manuals
QuikChange II XL Site-Directed Mutagenesis Kit (PDF)

Mutagenic primer design, mutant strand synthesis reaction, thermal cycling, Dpn I digestion, transformation of XL10-Gold Ultracompetent Cells, transformation guidelines.

  • Publication Part Number: 200521-12
  • Created: 08 Sep 2023
  • 333 KB
  • PDF
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User Manuals
QuikChange XL Site-Directed Mutagenesis Kit (PDF)

Materials, mutagenic primer design, mutant strand synthesis reaction, Dpn1 digestion, transformation of XL10-Gold Ultracompetent Cells.

  • Publication Part Number: 200516-12
  • Created: 08 Sep 2023
  • 414 KB
  • PDF
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User Manuals
QuikChange II Site-Directed Mutagenesis Kit (PDF)

Mutagenic primer design, mutant strand synthesis reaction, thermal cycling, Dpn I digestion of amplification products, transformation of XL1-Blue Supercompetent Cells.

  • Publication Part Number: 200523-12
  • Created: 08 Sep 2023
  • 320 KB
  • PDF
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User Manuals
QuikChange Lightning Multi Site-Directed Mutagenesis Kit (academic) (PDF)

Instruction manual for the academic version of QuikChange Lightning Multi Site-Directed Mutagenesis Kit with introduction, guidelines and protocol requirements.

  • Publication Part Number: 210513-12
  • Created: 08 Sep 2023
  • 487 KB
  • PDF
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User Manuals
QuikChange Site-Directed Mutagenesis Kit (PDF)

Materials, mutagenic primer design, mutant strand synthesis reaction, Dpn I digestion of amplification products, transformation of XL1-Blue Supercompetent Cells.

  • Publication Part Number: 200518-12
  • Created: 08 Sep 2023
  • 403 KB
  • PDF
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User Manuals
QuikChange II-E Site-Directed Mutagenesis Kit (PDF)

QuikChange II-E Site-Directed Mutagenesis Kit

  • Publication Part Number: 200555-12
  • Created: 08 Sep 2023
  • 369 KB
  • PDF
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User Manuals
GeneMorph II EZClone Domain Mutagenesis Kit (PDF)

How to eliminate bias and easily control mutation rate, EZclone reaction, mutant megaprimer synthesis, transformation guidelines, troubleshooting, and reagent preparation.

  • Publication Part Number: 200552-12
  • Created: 16 May 2023
  • 367 KB
  • PDF
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User Manuals
QuikChange HT Protein Engineering System Protocol (PDF)

QuikChange HT Protein Engineering System Protocol

  • Publication Part Number: G5900-90000
  • Created: 06 Oct 2014
  • 602 KB
  • PDF

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