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Parallel capillary electrophoresis system used for nucleic acid QC in low- to medium-throughput labs.
This document includes system overviews, analytical methods, maintenance procedures, software operation, troubleshooting guide, and instrument shutdown procedures.
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This technical overview details the different methods available for the analysis of IVT RNA using the HS RNA kit on the Fragment Analyzer.
This application note provides an example of a suitability study using the Agilent Fragment Analyzer system.
This technical overview provides examples of CQAs for IVT mRNA, such as size, concentration, purity, and integrity, that can be addressed by the Agilent Fragment Analyzer system.
This application note uses the Fragment Analyzer and HS RNA kit and provides an example of the calculation of encapsulation efficiency by quantifying IVT mRNAs before and after LNP disruption.
The compendium showcases Agilent Fragment Analyzer, TapeStation, 1290 Infinity Bio LC, and 6545 XT Advance Bio LC/Q-TOF systems in IVT mRNA biotherapeutics workflow.
In this technical overview, best practices for analysis of IVT mRNA are discussed, with particular attention to the use of the Agilent RNA kit and Fragment Analyzer systems.
The Agilent 5200 Fragment Analyzer provides sensitive, reliable quality control analysis of RNA in the in vitro transcription (IVT) RNA workflow.
This technical overview discusses best handling practices for IVT mRNA and analysis with the Fragment Analyzer systems, including sample handling and quantification tips.
This application note describes the procedures necessary to obtain reliable sizing for poly(A) tails using the Fragment Analyzer.
The use of in vitro transcription (IVT) mRNA is becoming widespread in research areas such as ribozyme and aptamer synthesis, mRNA synthesis, RNA interference, and antisense RNA techniques. Longer RNA transcripts greater than 3,000 nt are needed for...
The Agilent Fragment Analyzer and associated RNA Kits allow for easy analysis of RNA quality in RT-PCR, microarray analysis, and next-generation sequencing.
The Agilent 5200 Fragment Analyzer and associated RNA Kits allow for easy analysis of total RNA quality and concentration.
The Agilent 5200 Fragment Analyzer system provides high resolution separation and consistent sizing of the Cas9 mRNA with or without polyadenylation
High-throughput analysis for detection of CRISPR induced Homology Directed Repair (HDR) knock-in of novel restriction enzyme sites
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Visual sgRNA integrity quality assessment with separation of the linear and secondary structure fragments on the Agilent 5200 Fragment Analyzer system
Agilent Automated Capillary Electrophoresis Systems General Safety Guide
This application note introduces a method for target capture (long-read) sequencing using the Agilent SureSelect XT HS2 target enrichment system with some protocol modifications.
The Agilent 5200 Fragment Analyzer system provides reliable sizing for fragments and smears for many nucleic acid applications