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For blocking non-specific background staining in immunohistochemical procedures. Is compatible with all primary and secondary antibodies regardless of species.
Protein standards for aqueous GPC/SEC system and column calibration, available as kits
Protein A is native from S. aureus, binding with high affinity to the Fc region of immunoglobulins
Automate the CE-SDS analysis of denatured proteins with capillary electrophoresis.
The BCL6 gene encodes a 706 amino acid nuclear protein of the Kruppel-type zinc finger protein. It is rearranged in about 30% of diffuse large B-cell lymphomas, and is expressed predominantly in normal germinal centre B cells and related...
Automated Protein and Peptide Sample Preparation Tutorial Session
Automated Protein and Peptide Sample Preparation Tutorial Session
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The AssayMAP platform is powerful out of the box sample prep solution for automated protein and peptide characterization and quantification workflows such as intact mass analysis, peptide quantification, peptide mapping, post-translational modifications, and many more.
Measurement of protein-containing E+L migrates with ICP-MS
Measurement of protein-containing E+L migrates with ICP-MS
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Hello, we have to determine 33 elements in E+L migrates using ICP-MS. Many of these E+L migrates contain proteins. After diluting the E+L migrates with an aqueous 2% HNO3/1% HCl solution (1:10), the proteins precipitate. Apart from digestion with nitric acid/hydrochloric acid (microwave), are there any other options for measuring these E+L migrates without problems?
AssayMAP Bravo Protein Sample Preparation Platform — Resources
AssayMAP Bravo Protein Sample Preparation Platform — Resources
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Support and educational resources for the AssayMAP Bravo. Product Pages AssayMAP Bravo Protein Sample Preparation Platform Includes an overview of the AssayMAP system, literature, videos, and more AssayMAP cartridges With information about types of cartridges available and resin chemistry details Technology Explained AssayMAP Bravo Fundamentals webpage What AssayMAP Bravo Platform is and how it works FAQs to learn what the system can do Automated protein sample preparation that is easy to use Animated overview of the benefits of the AssayMAP Bravo Platform (1:42 min) Agilent AssayMAP BRAVO: For Protein and Peptide Sample Prep-subtitles Animated overview of the AssayMAP in motion (5:20 min) Unravelling the Myths of Lab Automation Video addressing common misperceptions about lab automation (3:13 min) Webinars and Videos Highlights: Automated Protein and Peptide Sample Prep in Regulated and Nonregulated Labs Just Got Easier Steve Murphy, Ph.D. from Agilent Technology (48:19 min) Overview of the AssayMAP Bravo and the capabilities of the AssayMAP Workbench 4.0 An Automated and Faster Affinity Capture Method for Biotransformation Assessment on Site Specific Antibody Drug Conjugates Aarti Jashnani, M.S. from BMS (20:18 min) Automated assay for MHC-bound peptide purification using AssayMAP Lichao Zhang, Ph.D. from Chan Zuckerberg Biohub (23:06 min) Automating Immunopeptidomics sample prep with the AssayMAP Bravo Automating MRM-Based Protein Quantification Tasso Miliotis, Ph.D. form AstraZeneca (46:59 min) Peeking Under the Hood of Cellular Signal Machinery with Targeted Proteomics Assays Jason Russell, Ph.D. from Agilent and Jacob Jaffe, Ph.D. from Broad Institute (57:54 min) Use of AssayMAP for Proteomics and Biologics Developability Presented by Mahmud Hossain, Ph.D. from Sanofi (21:21 min) See all AssayMAP webinars/videos Publications AssayMAP citation index is a complete list of peer-reviewed publications (<300) and app notes (<40).
Quantitation of protein on Agilent 6545XT instrument
Quantitation of protein on Agilent 6545XT instrument
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I have been working on improving the existing quantitative method for our vaccine project, which involves using TOF-MS EIC for surrogate peptides. My goal is to establish a pseudo-MRM (PRM) setup using a targeted MS/MS approach. This setup includes a fast TOF-MS scan with a targeted list of precursor ions for MS/MS, with an accumulation time of 20 ms per precursor ion. I would appreciate any advice or pointers on critical method parameters for setting up this method, as well as guidance on data processing tools and optimizing the processing method. I have started using the "Quant My Way" tool but would welcome any additional insights. Looking forward to your suggestions.
Reacts with the wild type and mutant type of the p53 protein. The antibody is a useful aid for classification of tumors of all cell lineages.
Labels MUM1 protein in a subset of light zone germinal centre (GC) B cells (probably centrocytes and their progeny), plasma cells and activated T cells. Antibodies to MUM1 protein are a useful aid for classification of a wide spectrum of...
Mismatch repair gene hMSH6 is a ubiquitous gene encoding the mismatch repair protein (MMR) known as MutS Protein Homolog 6 (MSH6). MSH6 is utilized by normal proliferating cells to repair point mutations that may occur during DNA replication....
Mismatch repair gene hMLH1 is a ubiquitous gene encoding the mismatch repair protein (MMR) known as MutL protein homolog 1 (MLH1). MLH1 is utilized by normal proliferating cells to repair point mutations that may occur during DNA replication....
Mismatch repair gene hMLH1 is a ubiquitous gene encoding the mismatch repair protein (MMR) known as MutL protein homolog 1 (MLH1). MLH1 is utilized by normal proliferating cells to repair point mutations that may occur during DNA replication....
p63 protein is a member of the p53 tumor suppressor family which also includes the p73 protein. These proteins act as transcription factors that regulate the progression of the cell through its cell cycle and cell death (apoptosis) in response to...
MutS protein homolog 6 (MSH6) is part of the mismatch repair (MMR) pathway which is utilized by normal proliferating cells to repair mutations that may occur during DNA replication. Antibodies to MSH6 are a useful aid for classification of tumors...
MutS protein homolog 6 (MSH6) is part of the mismatch repair (MMR) pathway which is utilized by normal proliferating cells to repair mutations that may occur during DNA replication. Antibodies to MSH6 are a useful aid for classification of tumors...
Labels MUM1 protein in a subset of light zone germinal centre (GC) B cells (probably centrocytes and their progeny), plasma cells and activated T cells. Antibodies to MUM1 protein are a useful aid for classification of a wide spectrum of...
The BCL6 gene encodes a 706 amino acid nuclear protein of the Kruppel-type zinc finger protein. It is rearranged in about 30% of diffuse large B-cell lymphomas, and is expressed predominantly in normal germinal centre B cells and related...
Mismatch repair gene hMLH1 is a ubiquitous gene encoding the mismatch repair protein (MMR) known as MutL protein homolog 1 (MLH1). MLH1 is utilized by normal proliferating cells to repair point mutations that may occur during DNA replication....
Mismatch repair gene hMSH2 is a ubiquitous gene encoding the mismatch repair protein (MMR) known as MutS protein homolog 2 (MSH2). MSH2 is utilized by normal proliferating cells to repair point mutations that may occur during DNA replication....
Reacts with the wild type and mutant type of the p53 protein. The antibody is a useful aid for classification of tumors of all cell lineages.
MutS protein homolog 2 (MSH2) is part of the mismatch repair (MMR) pathway which is utilized by normal proliferating cells to repair mutations that may occur during DNA replication. Antibodies to MSH2 are a useful aid for classification of tumors...
The Affinity protein expression and purification system is a simple, rapid, and effective method.